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Differential Levels of Alpha-2-Macroglobulin, Haptoglobin and Sero-Transferrin as Adjunct Markers for TB Diagnosis and Disease Progression in the Malnourished Tribal Population of Melghat, India

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Title Differential Levels of Alpha-2-Macroglobulin,
Haptoglobin and Sero-Transferrin as Adjunct
Markers for TB Diagnosis and Disease
Progression in the Malnourished Tribal
Population of Melghat, India
 
Creator Bapat, Prachi R
Satav, Ashish R
Husain, Aliabbas A
Shekhawat, Seema D
Kawle, Anuja P
Chu, Justin J
Purohit, H J
Daginawala, Hatim F
Taori, Girdhar M
Kashyap, Rajpal S
 
Subject Environmental Health
Microbiology
 
Description Lack of diagnostic capacity has been a crucial barrier preventing an effective response to
the challenges of malnutrition and tuberculosis (TB). Point-of-care diagnostic tests for TB in
immuno-incompetent, malnourished population are thus needed to ensure rapid and accurate
detection. The aim of the study was to identify potential biomarkers specific for TB
infection and progression to overt disease in the malnourished population of Melghat. A prospective
cohort study was conducted in the year 2009 through 2011 in six villages of the
Melghat region. 275 participants consisting of malnourished cases with a) active TB (n =
32), b) latent TB infection (n = 90), c) with no clinical or bacteriological signs of active or
latent TB (n = 130) and healthy control subjects (n = 23) were recruited for the study. The
proteome changes of the host serum in response to Mycobacterium tuberculosis (M.tb)
infection were investigated using one dimensional electrophoresis in combination with
matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF
MS). Three most differentially expressed proteins; alpha-2-macroglobulin (A-2-M), serotransferrin
and haptoglobin were identified by MALDI-TOF MS analysis, which were up-regulated
in the malnourished patients with active TB and down-regulated in the malnourished
patients compared with the healthy controls. Additionally, follow-up studies indicated that
the expression of these proteins increased to nearly two folds in patients who developed
active disease from latent state. Our preliminary results suggest that A-2-M, sero-transferrin
and haptoglobin may be clinically relevant host biomarkers for TB diagnosis and disease
progression in the malnourished population. This study provides preliminary framework for an in-depth analysis of the biomarkers in larger well-characterized cohorts. Evaluation of
these biomarkers in follow-up cases may further aid in improving TB diagnosis.
 
Publisher Public Library of Science
 
Date 2015-08-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/817/1/bapat%202015.pdf
Bapat, Prachi R and Satav, Ashish R and Husain, Aliabbas A and Shekhawat, Seema D and Kawle, Anuja P and Chu, Justin J and Purohit, H J and Daginawala, Hatim F and Taori, Girdhar M and Kashyap, Rajpal S (2015) Differential Levels of Alpha-2-Macroglobulin, Haptoglobin and Sero-Transferrin as Adjunct Markers for TB Diagnosis and Disease Progression in the Malnourished Tribal Population of Melghat, India. PLOS ONE. pp. 1-15. ISSN 1932-6203
 
Relation http://journals.plos.org/plosone/
http://neeri.csircentral.net/817/