Record Details

Dynamic interactive events in gene regulation using E. coli dehydrogenase as a model

IR@CSIR-NEERI

View Archive Info
 
 
Field Value
 
Title Dynamic interactive events in gene regulation
using E. coli dehydrogenase as a model
 
Creator Puranik , Sampada
Purohit, H J
 
Subject Microbiology
 
Description Different approaches in gene expression analysis
always provide a snapshot view of cellular events. During the
bacterial growth, the decisions are dynamically made with
participation of various genes and their interactions with modulating
factors. We have selected Escherichia coli dehydrogenases
as a model to capture these interactions. We have treated
the cells with hydrogen peroxide with very low level and
asked the questions how cellular physiology has modulated
itself to survive post-shock conditions. We hypothesized that
while global regulators and associated gene network dictate
the overall cellular intelligence, specific redox-sensitive classes
of enzymes like dehydrogenase-mediated modulation
could provide the option to cell for survival under peroxide
after-effect. To understand the dynamic gene interaction, we
used multidimensional scaling of genes and overlaid with
minimum spanning tree to understand the clustering patterns
under different conditions. Study shows that under peroxide
after-effect, it is the interplay of ArcA (global regulator), with
ldhA (involved in intermediary metabolism) and ndh (managing
co-factor NADH), that emerges as modulating association.
Knockout mutants of global regulators confirmed the promoter
activity trend through gene expression change for
dehydrogenases.
 
Publisher Springer
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/821/1/Puranik_2015.pdf
Puranik , Sampada and Purohit, H J (2015) Dynamic interactive events in gene regulation using E. coli dehydrogenase as a model. Functional & Integrative Genomics, 15 (2). pp. 175-188. ISSN 1438-793X, ESSN: 1438-7948
 
Relation https://link.springer.com/journal/10142
http://neeri.csircentral.net/821/