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Rapid Diagnosis and Simultaneous Identification of Tuberculous and Bacterial Meningitis by a Newly Developed Duplex Polymerase Chain Reaction

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Title Rapid Diagnosis and Simultaneous Identification of Tuberculous and Bacterial Meningitis by a Newly Developed Duplex Polymerase Chain Reaction
 
Creator Nagdev, Khushboo J
Bhagchandani, Sharda P
Bhullar, Shradha S
Kapgate, Rajkumar C
Kashyap, Rajpal S
Chandak, Nitin H
Daginawala, Hatim F
Purohit, H J
Taori, Girdhar M
 
Subject Microbiology
 
Description The present study describes the development
and evaluation of a duplex polymerase chain reaction (DPCR)
for diagnosis and simultaneous identification of
tuberculous meningitis (TBM) and bacterial meningitis
(BM) in a single reaction. A D-PCR with primers amplifying
portions of the Mycobacterium tuberculosis IS6110 and
the eubacteria 16SrDNA sequence in a same reaction mix
was developed and tested on DNA extracted from 150
clinical CSF samples from different categories (TBM = 39,
BM = 26, control infectious and non-infectious category
= 85). The results indicate a clear differentiation
between bands for eubacteria and M. tuberculosis with an
analytical sensitivity of 103 cfu/ml for eubacteria and
102 cfu/ml for M. tuberculosis. When evaluated in clinical
samples, D-PCR overall diagnosed 100 % confirmed TBM
and 100 % confirmed BM cases with overall specificity of
96.5 %. D-PCR can be an effective tool for diagnosis and
simultaneous identification of TBM or BM in a single PCR
reaction. It saves time, cost, labour and sample amount and
help in administration of appropriate antimicrobial therapy.
 
Publisher Springer
 
Date 2015-06
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/822/1/Nagdev_2015.pdf
Nagdev, Khushboo J and Bhagchandani, Sharda P and Bhullar, Shradha S and Kapgate, Rajkumar C and Kashyap, Rajpal S and Chandak, Nitin H and Daginawala, Hatim F and Purohit, H J and Taori, Girdhar M (2015) Rapid Diagnosis and Simultaneous Identification of Tuberculous and Bacterial Meningitis by a Newly Developed Duplex Polymerase Chain Reaction. Indian Journal of Microbiology, 55 (2). pp. 213-218. ISSN 0046-8991, ESSN: 0973-7715
 
Relation https://link.springer.com/journal/12088
http://neeri.csircentral.net/822/