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Novel bacteriophage therapy for controlling metallo-beta-lactamase producing Pseudomonas aeruginosa infection in Catfish

IR@CSIR-NEERI

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Title Novel bacteriophage therapy for controlling
metallo-beta-lactamase producing Pseudomonas
aeruginosa infection in Catfish
 
Creator Khairnar, Krishna
Raut, Mahendra P
Sanmukh, Swapnil G
Chandekar, Rajshree H
Paunikar, Waman N
 
Subject Microbiology
 
Description Background: The bacteriophage therapy is an effective antimicrobial approach with potentially important
applications in medicine and biotechnology which can be seen as an additional string in the bow. Emerging drug
resistant bacteria in aquaculture industry due to unrestricted use of antibiotics warrants more sustainable and
environmental friendly strategies for controlling fish infections.
The isolated bacteria from fish lesions was characterised based on isolation on selective and differential medium
like Pseudomonas agar, gram staining, biochemical tests and 16SrRNA sequencing. The metallo-beta-lactamase
(MBL) producing bacterial isolate was evaluated using Imipenem - Ethylenediaminetetraacetic acid (EDTA) disk
method. The specific bacteriophage was isolated and concentrated using coal bed developed in our lab at
CSIR-NEERI. The isolated and enriched bacteriophage was characterised by nucleotide sequencing and electron
microscopy. The phage therapy was applied for treating ulcerative lesion in fish.
Results: The pathogenic bacterium responsible for causing ulcerative lesions in catfish species (Clarias gariepinus)
was identified as Pseudomonas aeruginosa. One out of twenty P. aeruginosa isolate showing multi drug resistance
(MDR) was incidentally found to be MBL producing as determined by Imipenem-EDTA disk method. The phage
therapy effectively cured the ulcerative lesions of the infected fish in 8–10 days of treatment, with a sevenfold
reduction of the lesion with untreated infection control.
Conclusion: Bacteriophage therapy can have potential applications soon as an alternative or as a complement to
antibiotic treatment in the aquaculture. We present bacteriophage therapy as a treatment method for controlling
MDR P. aeruginosa infection in C. gariepinus. To the best of our knowledge this is a first report of application of
phage therapy against MBL producing P. aeruginosa isolated from aquatic ecosystem.
Keywords: P. aeruginosa, Multi drug resistance, Metallo-β-lactamase, Bacteriophage therapy, Catfish
 
Publisher BioMed Central
 
Date 2013-12-26
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/834/1/Publication_18.pdf
Khairnar, Krishna and Raut, Mahendra P and Sanmukh, Swapnil G and Chandekar, Rajshree H and Paunikar, Waman N (2013) Novel bacteriophage therapy for controlling metallo-beta-lactamase producing Pseudomonas aeruginosa infection in Catfish. BMC Veterinary Research, 9 (264). pp. 1-9. ISSN 1746-6148
 
Relation http://bmcvetres.biomedcentral.com/
http://neeri.csircentral.net/834/