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A simple and novel modification of comet assay for determination of bacteriophage mediated bacterial cell lysis

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Title A simple and novel modification of comet assay for determination of bacteriophage mediated bacterial cell lysis
 
Creator Khairnar, Krishna
Sanmukh, Swapnil
Chandekar, Rajshree
Paunikar, Waman
 
Subject Microbiology
 
Description The comet assay is the widely used method for in vitro toxicity testing which is also an alternative to the
use of animal models for in vivo testing. Since, its inception in 1984 by Ostling and Johansson, it is being
modified frequently for a wide range of application. In spite of its wide applicability, unfortunately there
is no report of its application in bacteriophages research. In this study, a novel application of comet assay
for the detection of bacteriophage mediated bacterial cell lysis was described. The conventional methods
in bacteriophage research for studying bacterial lysis by bacteriophages are plaque assay method. It is
time consuming, laborious and costly. The lytic activity of bacteriophage devours the bacterial cell which
results in the release of bacterial genomic material that gets detected by ethidium bromide staining
method by the comet assay protocol. The objective of this study was to compare efficacy of comet assay
with different assay used to study phage mediated bacterial lysis. The assay was performed on culture
isolates (N = 80 studies), modified comet assay appear to have relatively higher sensitivity and specificity
than other assay. The results of the study showed that the application of comet assay can be an economical,
time saving and less laborious alternative to conventional plaque assay for the detection of bacteriophage
mediated bacterial cell lysis.
 
Publisher Elsevier
 
Date 2014-02-21
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/835/1/Publication_21.pdf
Khairnar, Krishna and Sanmukh, Swapnil and Chandekar, Rajshree and Paunikar, Waman (2014) A simple and novel modification of comet assay for determination of bacteriophage mediated bacterial cell lysis. Journal of Virological Methods, 203. pp. 33-38. ISSN 0166-0934
 
Relation https://www.journals.elsevier.com/journal-of-virological-methods
http://neeri.csircentral.net/835/