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Overexpression of rice glutaredoxins (OsGrxs) significantly reduces arsenite accumulation by maintaining glutathione pool and modulating aquaporins in yeast

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Title Overexpression of rice glutaredoxins (OsGrxs) significantly reduces arsenite accumulation by maintaining glutathione pool and modulating aquaporins in yeast
 
Creator Verma, Pankaj Kumar
Verma, Shikha
Meher, Alok Kumar
Pande, Veena
Mallick, Shekhar
Bansiwal, Amit
Tripathi, Rudra Deo
Dhankher, Om Parkash
Chakrabarty, Debasis
 
Subject Environmental Biotechnology
 
Description Arsenic (As) is an acute poison and class I carcinogen, can cause a serious health risk. Staple crops like
rice are the primary source of As contamination in human food. Rice grown on As contaminated areas
accumulates higher As in their edible parts. Based on our previous transcriptome data, two rice glutaredoxins
(OsGrx_C7 and OsGrx_C2.1) were identified that showed up-regulated expression during As
stress. Here, we report OsGrx_C7 and OsGrx_C2.1 from rice involved in the regulation of intracellular
arsenite (AsIII). To elucidate the mechanism of OsGrx mediated As tolerance, both OsGrxs were cloned
and expressed in Escherichia coli (Dars) and Saccharomyces cerevisiae mutant strains (Dycf1, Dacr3). The
expression of OsGrxs increased As tolerance in E. coli (Dars) mutant strain (up to 4 mM AsV and up to
0.6 mM AsIII). During AsIII exposure, S. cerevisiae (Dacr3) harboring OsGrx_C7 and OsGrx_C2.1 have lower
intracellular AsIII accumulation (up to 30.43% and 24.90%, respectively), compared to vector control.
Arsenic accumulation in As-sensitive S. cerevisiae mutant (Dycf1) also reduced significantly on exposure
to inorganic As. The expression of OsGrxs in yeast maintained intracellular GSH pool and increased
extracellular GSH concentration. Purified OsGrxs displays in vitro GSH-disulfide oxidoreductase, glutathione
reductase and arsenate reductase activities. Also, both OsGrxs are involved in AsIII extrusion by
altering the Fps1 transcripts in yeast and protect the cell by maintaining cellular GSH pool. Thus, our
results strongly suggest that OsGrxs play a crucial role in the maintenance of the intracellular GSH pool
and redox status of the cell during both AsV and AsIII stress and might be involved in regulating
intracellular AsIII levels by modulation of aquaporin expression and functions.
 
Publisher Elsevier
 
Date 2016-09
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/843/1/Plant%20Physiology-NBRI.pdf
Verma, Pankaj Kumar and Verma, Shikha and Meher, Alok Kumar and Pande, Veena and Mallick, Shekhar and Bansiwal, Amit and Tripathi, Rudra Deo and Dhankher, Om Parkash and Chakrabarty, Debasis (2016) Overexpression of rice glutaredoxins (OsGrxs) significantly reduces arsenite accumulation by maintaining glutathione pool and modulating aquaporins in yeast. Plant Physiology and Biochemistry, 106. pp. 208-217. ISSN 0981-9428
 
Relation https://www.journals.elsevier.com/plant-physiology-and-biochemistry
http://neeri.csircentral.net/843/