Record Details

Genetic polymorphism of CYP2D6*2 C? T 2850, GSTM1, NQO1 genes and their correlation with biomarkers in manganese miners of Central India

IR@CSIR-NEERI

View Archive Info
 
 
Field Value
 
Title Genetic polymorphism of CYP2D6*2 C? T 2850, GSTM1, NQO1 genes
and their correlation with biomarkers in manganese miners
of Central India
 
Creator Vinayagamoorthy, Nadimuthu
Krishnamurthi, Kannan
Devi, Saravana S
Naoghare, Pravin K
Biswas, Raka
Biswas, Arup R
Pramanik, Sreemanta
Shende, Ashok R
Chakrabarti, Tapan
 
Subject Environmental Health
 
Description Manganese (Mn) intoxication is most often regarded as an occupational manifestation and occurs in
places such as manganese mines, dry cell battery plants and ceramic industries. In the present study,
the influence of genetic polymorphism in cytochrome P450 2D6 (CYP2D6*2), glutathione S-transferase
M1 (GSTM1) and NAD(P)H quinone oxidoreductase 1 (NQO1) genes on blood manganese and plasma prolactin
concentrations in manganese miners was investigated. Genotyping of CYP2D6*2 C ? T 2850 and
NQO1 C ? T 609 was carried out using polymerase chain reaction–restriction fragment length polymorphism
(PCR–RFLP) whereas the genotyping of GSTM1 was carried out by multiplex PCR using beta globin
as an internal control. Manganese miners with CYP2D6*2 C ? T 2850 variant genotype had relatively low
Mn concentration [GM: 21.4 ± 8.9 lg L1
] than the subjects with wild (GM: 36.3 ± 8.5 lg L1
) and heterozygous
(GM: 34.4 ± 6.9 lg L1
) genotypes. Miners with CYP2D6*2 variant genotypes showed low prolactin
levels (GM: 13.13 ± 1.6 ng mL1
) compared to the wild (GM: 16.4.4 ± 1.5 lg L1
) and heterozygous
(GM: 18.7 ± 1.6 ng mL1
) genotypes. Gene–gene interaction studies also revealed that the subjects with
CYP2D6*2 C ? T 2850 variant genotypes had low levels of Mn and prolactin. Our new findings suggest
that CYP2D6*2 C ? T 2850 variant genotypes can regulate plasma prolactin levels in manganese miners
of Central India and could be involved in the fast metabolism of blood manganese, compared to wild and
heterozygous genotypes.
201
 
Publisher Elsevier
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/859/1/VinayagamoorthyetalChemosphere_2010.pdf
Vinayagamoorthy, Nadimuthu and Krishnamurthi, Kannan and Devi, Saravana S and Naoghare, Pravin K and Biswas, Raka and Biswas, Arup R and Pramanik, Sreemanta and Shende, Ashok R and Chakrabarti, Tapan (2010) Genetic polymorphism of CYP2D6*2 C? T 2850, GSTM1, NQO1 genes and their correlation with biomarkers in manganese miners of Central India. Chemosphere, 81. pp. 1286-1291. ISSN 0045-6535, ESSN: 1879-1298
 
Relation https://www.journals.elsevier.com/chemosphere/
http://neeri.csircentral.net/859/