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Influence of CO 2 concentration on carbon concentrating mechanisms in cyanobacteria and green algae: a proteomic approach

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Title Influence of CO 2 concentration on carbon concentrating mechanisms in cyanobacteria and green algae: a proteomic approach
 
Creator Ramanan, Rishiram
Vinayagamoorthy, Nadimuthu
Devi , S S
Krishnamurthi, Kannan
Chakrabarti, Tapan
 
Subject Environmental Biotechnology
 
Description Carbon concentrating mechanisms play a vital role in photosynthesis in microalgae and cyanobacteria especially in the proper functioning of Rubisco and assimilation of carbon via the Calvin cycle. This study evaluates the role of carbon dioxide on carbon concentrating mechanism (CCM) in a cynaobacteria, Spirulina platensis and a microalga, Chlorella sp. 786. The study organisms were grown in both atmospheric (control sample, 0.035%) and high (exposed sample, 10%) CO2 concentrations. Second dimension (2D) electrophoresis revealed a huge difference in the protein profiles of both organisms suggesting the induction of CCM related proteins in the sample maintained at atmospheric CO2 concentration and the repression of CCM related proteins in the sample maintained at 10% CO2. Liquid chromatography-mass spectroscopy analysis revealed the presence of two important Ci transporter proteins in the control sample of S. platensis, namely ferredoxin-NADP+ reductase and ATP binding cassette (ABC) transport system protein. These proteins were only expressed in the control sample and were downregulated or not expressed at all in the exposed sample. Consequently, this study conclusively proves that CCMs are only inducted at low CO2 concentrations and are not functional at high CO2 concentration.
 
Publisher The Korean Society of Phycology
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/918/1/Influence%20of%20CO2%20concentration%20on%20carbon%20concentrating%20mechanisms.pdf
Ramanan, Rishiram and Vinayagamoorthy, Nadimuthu and Devi , S S and Krishnamurthi, Kannan and Chakrabarti, Tapan (2012) Influence of CO 2 concentration on carbon concentrating mechanisms in cyanobacteria and green algae: a proteomic approach. Algae, 27 (4). pp. 295-301. ISSN 1226-2617
 
Relation http://koreascience.or.kr/article/ArticleFullRecord.jsp?cn=JORHBK_2012_v27n4_295
http://neeri.csircentral.net/918/