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Antigenotoxic potential of Gymnema montanum leaves on DNA damage in human peripheral blood lymphocytes and HL-60 cell line

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Title Antigenotoxic potential of Gymnema montanum leaves on DNA damage in human peripheral blood lymphocytes and HL-60 cell line
 
Creator Ramkumar, K M
Sankar, L
Manjula, C
Krishnamurthi, Kannan
Devi, S S
Chakrabarti, T
Kalaiselvi, K
Palanivel, M
Rajaguru, P
 
Subject Biochemistry
 
Description In this study we have evaluated the genoprotective
effect of the ethanol extract of Gymnema montanum
(GLEt) leaves in human peripheral blood lymphocytes
and HL-60 cell line in vitro using the
comet assay. DNA damage was induced by treating
the cells with H2O2 and methyl methane
sulphonate (MMS). GLEt treatment effectively protected
the lymphocytes and HL-60 cell line from
H2O2-induced oxidative DNA damage in a dosedependent
manner whereas it was not effective
against alkylative DNA damage caused by MMS.
The global percent repair efficiency also showed
that both pre- and post- GLEt treatment provided
effective protection against H2O2 induced DNA
damage but not as effective against MMS. At
200 lg ml21 level, its repair capacity against
H2O2 induced DNA damage was comparable to
that of vitamin-C (100 lM). Furthermore, exposure
to GLEt reduced the formation of apoptotic cells
caused by H2O2, which was demonstrated by the
decreased sub-G1-DNA content in cell cycle analysis
and apoptotic frequencies of lymphocytes in
an annexin-V binding assay. In addition, GLEt
was found to have effective peroxide scavenging
ability in dose-dependent manner. The protective
efficiency of the extract was found to be directly
proportional to its total phenolic content. The present
study indicates that G. montanum leaves are a
significant source of phytochemicals with antigenotoxic
and antioxidant activity, and thus has
potential therapeutic use. Environ. Mol. Mutagen
 
Publisher Wiley
 
Date 2010-05
 
Type Article
NonPeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/935/2/Ramkumar_et_al-2010-Environmental_and_Molecular_Mutagenesis.pdf
Ramkumar, K M and Sankar, L and Manjula, C and Krishnamurthi, Kannan and Devi, S S and Chakrabarti, T and Kalaiselvi, K and Palanivel, M and Rajaguru, P (2010) Antigenotoxic potential of Gymnema montanum leaves on DNA damage in human peripheral blood lymphocytes and HL-60 cell line. Environmental and Molecular Mutagenesis, 51 (4). pp. 285-293. ISSN 0893-6692, ESSN: 1098-2280
 
Relation http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1098-2280/
http://neeri.csircentral.net/935/