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Single enzyme nanoparticle for biomimetic CO2 sequestration

IR@CSIR-NEERI

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Title Single enzyme nanoparticle for biomimetic CO2
sequestration
 
Creator Yadav, Renu
Labhsetwar, Nitin
Kotwal, Swati
Rayalu, Sadhana
 
Subject Materials Science
 
Description Nanoparticle technology is being increasingly
used in environmental sciences. We prepared
single enzyme nanoparticle (SEN) by modifying the
surface of carbonic anhydrase (CA) with a thin layer of
organic/inorganic hybrid polymer. SEN-CA appears to
be improving the stability of free enzyme. CA, as
ubiquitously found enzyme, is involved in gaseous
CO2 sequestration and is being looked as a promising
candidate for combating global warming. We report
here physical characterization of SEN-CA using
transmission electron microscope (TEM), Fouriertransform
infrared analysis (FTIR), X-ray diffraction
analysis (XRD), and energy dispersive X-ray (EDX).
Average size of SEN-CA particles appears to be in the
range of 70–80 nm. We also report the effect of SEN
formation on the kinetic parameters of free CA such as
Michaelis–Menten constant (Km), maximum reaction
velocity (Vmax), and storage stability of free CA and
SEN-CA. The Vmax of SEN-CA (0.02857 mmol/min/
mg) and free enzyme (0.02029 mmol/min/mg) is
almost similar. Km has decreased from 6.143 mM for
SEN-CAto 1.252 mMfor free CA. The stabilization of
CA by SEN formation results in improved the half-life
period (up to 100 days). The formation of carbonate
was substantiated by using gas chromatography (GC).
The conversion of CO2 to carbonate was 61 mg of
CaCO3/mg of CA and 20.8 mg of CaCO3/mg of CA
using SEN-CA and free CA, respectively.
 
Publisher Springer
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1037/1/yadav2011.pdf
Yadav, Renu and Labhsetwar, Nitin and Kotwal, Swati and Rayalu, Sadhana (2011) Single enzyme nanoparticle for biomimetic CO2 sequestration. Journal of Nanoparticle Research, 13 (1). pp. 263-271. ISSN 1388-0764 ESSN: 1572-896X)
 
Relation https://link.springer.com/journal/11051
http://neeri.csircentral.net/1037/