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You have full text access to this contentMembrane filtration immobilization technique—a simple and novel method for primary isolation and enrichment of bacteriophages

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Title You have full text access to this contentMembrane filtration immobilization technique—a simple and novel method for primary isolation and enrichment of bacteriophages
 
Creator Ghugare, G S
Nair, A
Nimkande, V
Sarode, P
Rangari, P
Khairnar, Krishna
 
Subject Environmental Biotechnology
Microbiology
 
Description Aim: To develop a method for the isolation and enrichment of bacteriophages
selectively against specific bacteria coupled with a membrane filtration technique.
Methods and Results: Rapid isolation and concentration of host-specific
bacteriophages was achieved by exposure of the sample suspected to contain
bacteriophages to a specific host immobilized on a 0�45 lm membrane in a
membrane filtration unit. The principle behind this method is the exploitation
of host-specific interaction of bacteriophages with their host and maximizing
this interaction using a classic membrane filtration method. This provides a
chance for each bacteriophage in the sample to interact with the specific host
on the membrane filter fitted with a vacuum pump. Specific bacteriophages of
the host are retained on the membrane along with its host cells due to the
effect of adsorption and these adsorbed bacteriophages (along with their hosts)
on the filter disc are then amplified and enriched in regular nutritive broth
tryptose soya broth by incubation. With the help of the plaque assay method,
host-specific phages of various bacterial species were isolated, segregated and
enriched.
Conclusions: The phage concentration method coupled with membrane
filtration immobilization of host bacteria was able to isolate and enrich the
host-specific bacteriophages by several fold using a lower quantity of an
environmental water sample, or other phage suspensions. Enrichment of
phages from single plaques was also achieved.
Significance and Impact of the Study: The isolation and detection of hostspecific
bacteriophages from a low density bacteriophage water sample in a
single step by the use of a simple and basic microbiological technique can be
achieved. Enrichment of phages from low phage titre suspensions is also
achieved very effectively.
 
Publisher Wiley
 
Date 2017
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1038/1/Ghugare_et_al-2017-Journal_of_Applied_Microbiology.pdf
Ghugare, G S and Nair, A and Nimkande, V and Sarode, P and Rangari, P and Khairnar, Krishna (2017) You have full text access to this contentMembrane filtration immobilization technique—a simple and novel method for primary isolation and enrichment of bacteriophages. Journal of Applied Microbiology, 122 (2). pp. 531-539. ISSN 1364-5072
 
Relation http://onlinelibrary.wiley.com/doi/10.1111/jam.13344/full
http://neeri.csircentral.net/1038/