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Integrative genome-wide association studies (GWAS) to understand complex genetic architecture of quantitative traits in chickpea

OAR@ICRISAT

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Relation http://oar.icrisat.org/10288/
 
Title Integrative genome-wide association studies (GWAS) to understand complex genetic architecture of quantitative traits in chickpea
 
Creator Parida, S K
Kujur, A
Bajaj, D
Das, S
Srivastava, R.
Badoni, S
Laxmi, -
Thakro, V
Basu, U
Daware, A
Upadhyaya, H D
Tripathi, S
Bharadwaj, C
 
Subject Chickpea
Genetics and Genomics
 
Description Development of high-yielding stress-tolerant chickpea cultivars
is essential to enhance its yield potential and productivity amidst
climate change scenario. Unfortunately, superior lines/recombinants
producing higher pod and seed yield under stress are not
available in world chickpea collection. Therefore, genetic dissection
of complex stress tolerance and yield-contributing quantitative
traits is the prime objective in current chickpea genomics
and breeding research. Our study employed diverse GWAS-assisted
integrated genomic strategies involving classical genetic
inheritance analysis, QTL mapping, differential transcript profiling,
molecular haplotyping and haplotype-based gene domestication/
evolution study for rapid quantitative dissection of complex
yield and stress tolerance traits in chickpea. To accomplish
this, multi-location/years replicated yield traits-related field
phenotyping and high-throughput marker genotyping information
generated from numerous natural germplasm accessions
(association panel) and multiple intra- and inter-specific mapping
populations of chickpea were deployed in the aforesaid
combinatorial genomic approaches. These analyses delineated
12 novel alleles and six haplotypes from 10 transcription factor
genes and 16 major QTLs/eQTLs governing yield and stress tolerance
traits that were mapped on 10 ultra-high density chickpea
genetic linkage maps. The superior natural alleles/haplotypes of
two major genes (QTLs) regulating seed weight and pod/seed
number identified from cultivated and wild Cicer gene pools are
being introduced into multiple high-yielding Indian varieties of
chickpea for its marker-assisted genetic improvement. The potential
molecular signatures delineated using integrated genomics-
assisted breeding strategies have functional significance to
understand the molecular genetic mechanism and natural allelic
diversity-led domestication pattern underlying these complex
quantitative traits at a genome-wide scale, leading to fast-paced
translational genomics for chickpea genetic enhancement.
These essential outcomes will be useful for devising the most
efficient strategies to produce high-yielding climate-resilient
chickpea cultivars for sustaining global food security.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/10288/1/Abstract_Book_114.pdf
Parida, S K and Kujur, A and Bajaj, D and Das, S and Srivastava, R. and Badoni, S and Laxmi, - and Thakro, V and Basu, U and Daware, A and Upadhyaya, H D and Tripathi, S and Bharadwaj, C (2017) Integrative genome-wide association studies (GWAS) to understand complex genetic architecture of quantitative traits in chickpea. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.