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Diet-induced hypercholesterolemia alters liver glycosaminoglycans and associated-lipoprotein receptors in rats.

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Relation http://ir.cftri.com/13139/
http://dx.doi.org/10.1007/s13105-017-0583-z
 
Title Diet-induced hypercholesterolemia alters liver
glycosaminoglycans and associated-lipoprotein receptors in rats.
 
Creator Kiran, G.
Prasada Rao, U. J. S.
Salimath, P. V.
Nandini, C. D.
 
Subject 28 Polysaccharide Chemistry
04 Diabetes Mellitus
 
Description Glycosaminoglycans (GAGs) play an important
role in lipoprotein metabolism. In liver, it facilitates the
uptake of remnants through receptor-independent endocytosis.
However, changes in liver GAGs during diet-induced
hypercholesterolemia with normal levels of fat feeding are
unknown. Present paper highlights the effect of dietinduced
hypercholesterolemia with normal levels (5%) of
fat on liver GAGs and other associated lipoprotein receptors.
Hypercholesterolemia was induced in rats by feeding
diet supplemented with 0.5% cholesterol and 0.125% bile
salts. Hypercholesterolemia showed significantly decreased
GAGs of both heparan sulfate (HS) and chondroitin
sulfate/dermatan sulfate (CS/DS) classes of molecules.
Quantitative real-time polymerase chain reaction analysis
of GAG biosynthetic enzymes and other genes revealed
significant changes in expression profile. The decrease in
GAGs was prevented by simvastatin treatment; a drug that
inhibits endogenous cholesterol synthesis that was used as
a positive control in our study. Furthermore, there was a
comparatively decreased binding of GAGs from hypercholesterolemic
rats to lipoprotein lipase. LRP1 which plays a
major role in lipoprotein uptake was also significantly decreased,
and it was attenuated in simvastatin-treated hypercholesterolemic
rats. Furthermore, LDLR and ApoE were
also decreased significantly in liver of hypercholesterolemic
rats. Thus, diet-induced hypercholesterolemia results in dysregulation
of cholesterol homeostasis apparently through
changes in GAGs in conjunction with other associated
players.
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13139/1/J%20Physiol%20Biochem%20%282017%29%2073539%E2%80%93550.pdf
Kiran, G. and Prasada Rao, U. J. S. and Salimath, P. V. and Nandini, C. D. (2017) Diet-induced hypercholesterolemia alters liver glycosaminoglycans and associated-lipoprotein receptors in rats. Journal of Physiology and Biochemistry, 73. pp. 539-550.