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Structural and functional characterization of a novel immunomodulatory glycoprotein isolated from ajowan (Trachyspermum ammi L.).

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Relation http://ir.cftri.com/13140/
http://dx.doi.org/10.1007/s10719-017-9771-x
 
Title Structural and functional characterization of a novel
immunomodulatory glycoprotein isolated from ajowan
(Trachyspermum ammi L.).
 
Creator Shruthi, R. R.
Venkatesh, Y. P.
Muralikrishna, G.
 
Subject 30 Spices/Condiments
 
Description Ajowan (Trachyspermum ammi L.) spice has been
used in food preparations and also as a traditional medicine in
Ayurveda. Although a number of pharmacological activities
have been attributed to ajowan, its role in immunomodulation
is not known. The main objective of the present study is to
examine the macromolecular immunomodulatory components.
Macrophage activation was studied by nitric oxide
(NO) release, phagocytosis and secretion of proinflammatory
cytokines as the markers. Ethanol precipitate
(fractional) of ajowan aqueous extract was subjected to conventional
chromatography (Q Sepharose followed by Bio-Gel
P-100). One of the proteins (30.7 kDa; ajowan glycoprotein or
Agp) showed effective mitogenic activity towards
splenocytes. Agp is a O-linked glycoprotein with the glycans
contributing to one-third of the molecular mass. It has a high
content of glutamic acid, serine, aspartic acid and proline
whereas galactose (45.7%), arabinose (34.5%), glucose
(7%), mannose (5%) and xylose (4%) are the constituent
sugars. Secondary structure analysis indicated that Agp contains
79% α-helices and 21% random coil. Internal sequencing
of the tryptic peptides did not show homology with the
existing proteins in the database (BLAST). Agp at 1 μg/mL
induced proliferation of B-cell enriched murine splenocytes
and activated macrophages in releasing NO and promoted
phagocytosis (p < 0.01). RAW 264.7 cells produced proinflammatory
cytokines (IL-12, TNF-α and IFN-γ) at 1 μg/
mL Agp (p < 0.01). Deproteinized Agp (dpAgp) failed to elicit
activation of murine immune cells, whereas deglycosylated
Agp (20 kDa; dgAgp) showed compromised efficiency. This
is the first report of an immunomodulatory protein from
ajowan.
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13140/1/Glycoconj%20J%20%282017%29%2034499%E2%80%93514.pdf
Shruthi, R. R. and Venkatesh, Y. P. and Muralikrishna, G. (2017) Structural and functional characterization of a novel immunomodulatory glycoprotein isolated from ajowan (Trachyspermum ammi L.). Glycoconjugate Journal, 34. pp. 499-514. ISSN 0282-0080