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Fine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed weight and drought related traits in chickpea

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Relation http://oar.icrisat.org/10304/
 
Title Fine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed
weight and drought related traits in chickpea
 
Creator Kale, S M
Jaganathan, D
Roorkiwal, M
Thudi, M
Purushothaman, R
Praveenkumar, M
Krishnamurthy, L
Kavi Kishor, P B
Gaur, P M
Varshney, R K
 
Subject Abiotic Stress
Drought Tolerance
Chickpea
Drought
Genetics and Genomics
 
Description A “QTL-hotspot” region of 7 Mb size for drought component traits
was identified on CaLG04, using a recombinant inbred line (RIL)
population (ICC 4958 × ICC 1882) in chickpea. Further, skimbased
genotyping by sequencing (GBS) approach with large SNP
markers delimited the “QTL-hotspot” region into two sub-regions;
viz, “QTL-hotspot_a” of 139.22 kb and “QTL-hotspot_b” of 153.36
kb, on the kabuli draft genome sequence. In order to validate
and identify more recombinations in the sub-regions for further
refinement, a fine mapping population with 1,911 lines was developed.
Flanking markers of the two “QTL-hotspot” sub-regions
were converted to KASPar assays and used to screen the fine
mapping population consisting of 1,911 lines. As a result, 19 F2:3
recombinant families were identified. These families were phenotyped
for seed weight and other drought-related traits. Comparison
of genotype and phenotype data identified a genomic region
of ~113 Kb size within “QTL-hotspot_a” responsible for 100 seed
weight (100SDW) and other drought-related traits in chickpea.
Subsequently, a syntenic study between the refined “QTL-hotpsot”
region and desi genome identified a 2 Mb region on Ca_LG_4
pseudomolecule. Whole genome re-sequencing (WGRS) analysis
of selected lines from each recombinant family identified
several non-synonymous and InDel mutations within important
candidate genes. Functional validation of these genes will help
decipher the mechanism of drought stress tolerance in chickpea.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/10304/1/Abstract_Book_402.pdf
Kale, S M and Jaganathan, D and Roorkiwal, M and Thudi, M and Purushothaman, R and Praveenkumar, M and Krishnamurthy, L and Kavi Kishor, P B and Gaur, P M and Varshney, R K (2017) Fine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed weight and drought related traits in chickpea. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.