Record Details

High throughput phenotyping and advanced genotyping reveals QTLs for plant vigor and water saving traits in a “QTL-hotspot”: New opportunities for enhancing drought tolerance in chickpea

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/10305/
 
Title High throughput phenotyping and advanced genotyping reveals QTLs for plant
vigor and water saving traits in a “QTL-hotspot”: New opportunities for enhancing drought
tolerance in chickpea
 
Creator Sivasakthi, K
Thudi, M
Tharanya, M
Kale, S M
Kholova, J
Halime, M H
Jaganathan, D
Baddam, R
Thirunalasundari, T
Gaur, P M
Varshney, R K
Vadez, V
 
Subject Abiotic Stress
Drought Tolerance
Chickpea
Drought
Genetics and Genomics
 
Description Terminal drought stress leads to substantial yield losses in
chickpea (Cicer arietinum L.). Water conservation at vegetative
growth (canopy conductivity and canopy size and development)
allow plants to increase soil water extraction during grain filling
and are hypothesised to help chickpea adaptation to water
limited environments. Plant vigour and water saving traits were
phenotyped in 232 recombinant inbred lines (RILs), derived
from a cross between ICC4958 and ICC1882, at 28 days after
sowing under well water conditions using a high throughput
phenotyping platform. Different density genetic maps (241-SSRLow
density, 1007-SSR+SNPs-High density and 1557-SNPs-Ultra
high density) were used for QTLs identification. Several major
QTLs (M-QTLs) for plant vigour traits (3D-leaf area, shoot biomass,
plant height and growth related traits) were identified
on CaLG04, and co-mapped with previously identified and
fine mapped major drought tolerance QTL-hotspot region on
CaLG04 (~300Kb).The canopy conductance trait (e.g Transpiration
rate) had a M-QTL mapped on CaLG03 using ultra-high
density bin markers. Plant vigour traits on CaLG04 and canopy
conductance related traits on CaLG03 provide opportunity
to manipulate these loci to tailor recombinants having lower
transpiration rate and high plant vigour. This ideotype might be
enhancing the water stress adaptation in chickpea. To test this
hypothesis, a subset of 40 RILs contrasting for vigour and water
use traits was tested in lysimeters and field under different water
stress treatments. High vigour low water use lines had higher
seed yield under severe water stress treatments than high vigour
and high water use lines, validating the hypothesis.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/10305/1/Abstract_Book_402.pdf
Sivasakthi, K and Thudi, M and Tharanya, M and Kale, S M and Kholova, J and Halime, M H and Jaganathan, D and Baddam, R and Thirunalasundari, T and Gaur, P M and Varshney, R K and Vadez, V (2017) High throughput phenotyping and advanced genotyping reveals QTLs for plant vigor and water saving traits in a “QTL-hotspot”: New opportunities for enhancing drought tolerance in chickpea. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.