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Insights on host-pathogen interaction between groundnut (Arachis hypogaea) and Aspergillus flavus

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Relation http://oar.icrisat.org/10308/
 
Title Insights on host-pathogen interaction between groundnut (Arachis hypogaea) and Aspergillus flavus
 
Creator Nayak, S N
Agarwal, G
Pandey, M K
Sudini, H
Jayale, A S
Purohit, S
Bajaj, P
Desai, A
Wan, L
Guo, B
Liao, B
Varshney, R K
 
Subject Groundnut
Plant Disease
Aflatoxins
 
Description Aflatoxin contamination, caused by fungal pathogen Aspergillus
flavus, is the major quality and health problem delimiting the trade
and consumption of groundnut (Arachis hypogaea L.) worldwide.
Three types of aflatoxin resistance mechanisms namely, resistance
to in-vitro seed colonization (IVSC), pre-harvest aflatoxin
contamination (PAC) and aflatoxin production (AP) have been
reported in groundnut. Transcriptome sequencing approach was
used to study the differentially expressed genes that differ in-vitro
seed colonization (IVSC) in resistant (J 11) and susceptible
(JL 24) genotypes. A total of 1,344 million raw reads with an
average of 84 million reads per sample were generated from 16
libraries from four different stages of fungal infection. A total of
737.75 and 770.83 million reads were mapped on the progenitor
genomes- A subgenome (A. duranensis) and B subgenome (A.
ipaensis) of cultivated groundnut (A. hypogaea), respectively. In
groundnut, defense related genes like senescence associated
proteins, resveratrol synthase, seed linoleate 9s-lipoxygenases
(9s-LOX), pathogenesis related proteins, peroxidases, glutathione-
S-transferases, chalcone synthase, defensin and chitinases
were differentially expressed. In A. flavus, the genes involved
in growth and development of fungus, aflatoxin biosynthesis,
binding and transporter proteins were found to be induced in
compatible interaction. In addition to IVSC resistance, we have
also carried out transcriptome sequencing for PAC and AP resistance.
In summary, this study will provide greater insights on
the resistance mechanisms and discovery of candidate genes for
all the three mechanisms that can further be used as expression
markers in genomics-enabled aflatoxin resistance breeding.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10308/1/Abstract_Book_409.pdf
Nayak, S N and Agarwal, G and Pandey, M K and Sudini, H and Jayale, A S and Purohit, S and Bajaj, P and Desai, A and Wan, L and Guo, B and Liao, B and Varshney, R K (2017) Insights on host-pathogen interaction between groundnut (Arachis hypogaea) and Aspergillus flavus. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.