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Genome wide analysis of sodium transporters and expression of Na+/H+-antiporter-like protein (SbNHXLP) gene in tomato for salt tolerance

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Relation http://oar.icrisat.org/10309/
 
Title Genome wide analysis of sodium transporters and expression of Na+/H+-antiporter-like protein (SbNHXLP) gene in tomato for salt tolerance
 
Creator Palavalasa, H K
Narasu, L N
Varshney, R K
Kavi Kishor, P B
 
Subject Genetics and Genomics
 
Description Salt stress is perceived by plants as ionic and osmotic stresses.
Excess Na+ and Cl- instigate conformational changes in
protein architecture and membrane depolarization leading to
the ion toxicity perception. At cellular level, sodium toxicity
can be sensed by plasma ion transporters, membrane proteins,
and Na+ sensitive enzymes. In the present study, a full-length
cDNA homologue of salt overly sensitive1 (SbNHXLP) gene
was isolated from Sorghum bicolor, and its overexpression in tomato
plants lead to less Na+ and more K+accumulation in root
and flower tissues indicating that it helps in proper ion homeostasis.
Cambial activity and increased xylogenesis have been
noticed under salt stress in transgenic root and stem tissues
unlike that of untransformed controls. In silico protein-protein
interaction studies of NHXLP revealed hypothetical interactions
with several sodium proton antiporter (NHX and SOS)
and cation proton antiporter (CHX) proteins. Co-immunoprecipitation
followed by MALDI-TOF analysis showed that
NHXLP protein interacts in vitro with one of the cation proton
antiporter (CPA) family members CHX2, belonging to CPA1
family. Comparative genomics helped in knowing the evolutionary
history by studying the phylogenetic distance, divergence
and synteny of Sodium transporters between sorghum,
maize and rice. This information would be useful in selecting
candidate genes for functional validation in relation to abiotic
stress tolerance during various developmental phases in crop
species Thus, SbNHXLP gene appears to be associated with
Na+ exclusion at the plasma membrane and helps the tomato
transgenics to ameliorate salt stress.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10309/1/Abstract_Book_410.pdf
Palavalasa, H K and Narasu, L N and Varshney, R K and Kavi Kishor, P B (2017) Genome wide analysis of sodium transporters and expression of Na+/H+-antiporter-like protein (SbNHXLP) gene in tomato for salt tolerance. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.