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Next generation sequencing based transcriptomic studies for crop improvement in pigeonpea

OAR@ICRISAT

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Relation http://oar.icrisat.org/10310/
 
Title Next generation sequencing based transcriptomic studies for crop improvement in pigeonpea
 
Creator Pazhamala, L T
Saxena, R K
Purohit, S
Bajaj, P
Kumar, V
Garg, V
Srikanth, S
Hingane, A J
Kulshreshtha, A
Krishnamurthy, L
Sameer Kumar, C V
Verdier, J
Varshney, R K
 
Subject Pigeonpea
Genetics and Genomics
 
Description Transcriptomic studies are rapidly evolving as a powerful tool
with next-generation sequencing technology to understand gene
functions and molecular mechanisms. RNA sequencing (RNAseq)
provides a dynamic range for transcript detection and a
better quantification of expression levels.With the availability of
genome sequence in pigeonpea, RNA-seq was used to link the
sequence information to phenotypic traits resulting from specific
developmental processes. In pigeonpea, three-line hybrid
breeding system is well-established; however, it is technically
demanding and cumbersome. In order to explore the possibility
of a two-line hybrid breeding system, a coherent transcriptomic
approach supported by physiological and cytological data has
led to the identification of a temperature-sensitive male sterile
(TSMS) line. This line has been characterized for critical (tetrad)
stage and temperature (23°C), and the identification of candidate
genes involved in abscisic acid signaling for fertility reversion.
Furthermore, a gene expression atlas (CcGEA) has been developed
and transcriptomic profiles generated for studying pod
and seed development with a dataset of 590.84 and 342 million
paired-end reads, respectively in pigeonpea. These data have
been analyzed for genes with differential, specific, spatio-temporal
and constitutive expression. In addition, CcGEA identified a
gene network of 28 co-expressed genes, including two regulatory
genes, a pollen specific SF3 and a sucrose-proton symporter to
be involved in pollen fertility, which has potential implication in
seed yield improvement. In summary, this study, especially identification
of TSMS and development of CcGEA, will accelerate
on-going efforts to enhance genetic gains in pigeonpea.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10310/1/Abstract_Book_412.pdf
Pazhamala, L T and Saxena, R K and Purohit, S and Bajaj, P and Kumar, V and Garg, V and Srikanth, S and Hingane, A J and Kulshreshtha, A and Krishnamurthy, L and Sameer Kumar, C V and Verdier, J and Varshney, R K (2017) Next generation sequencing based transcriptomic studies for crop improvement in pigeonpea. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.