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Next generation sequencing approaches for understanding genetic mechanism of drought tolerance in Valencia Peanut

OAR@ICRISAT

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Relation http://oar.icrisat.org/10311/
 
Title Next generation sequencing approaches for understanding genetic mechanism of drought tolerance in Valencia Peanut
 
Creator Puppala, N
Xavier, A
Garg, V
Nayak, S N
Pandey, M K
Varshney, R K
Layland, N
Payton, P
Holbrook, C
 
Subject Abiotic Stress
Drought Tolerance
Genetic Engineering
Groundnut
Drought
Genetics and Genomics
 
Description Valencia peanuts are highly susceptible to drought stress.
The yield reduction due to stress can result in more than 50%
reduction resulting in high aflatoxin contamination. In our
present study we have compared Valencia-C genotype with
a highly drought tolerant runner type peanut C76-16 using
transcriptome sequencing to investigate tissue-specific gene
expression and response to abiotic stress. We evaluated the
two genotypes under field conditions under irrigated and stress
plots in 4 replications. We found that genotype C76-16 outperformed
yield compared to Valencia-C under drought stress
conditions with only a 30 % reduction in yield while Valencia-C
showed a higher loss of up to 70%. Each year the yield varied
widely due to the environmental factors but C76-16 was consistently
ranked among the top and suffered a minimal loss of
yield suggesting that it is best suited for the erratic climatic
conditions of southwest U.S. Leave samples were harvested
from the control and stress conditions and used for RNA isolation
and sequencing. A total of 340 million paired-end reads
were generated and were used to find differentially expressed
genes and transcripts between tolerant and susceptible genotypes.
The pathways and candidate genes involved in drought
tolerance will provide basic information and expression markers
for drought tolerance in peanut. We will present our findings
on genic content and tissue-specific gene expression and
discuss the challenges and opportunities of unifying transcript
sequence data for the peanut community.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/10311/1/Abstract_Book_413.pdf
Puppala, N and Xavier, A and Garg, V and Nayak, S N and Pandey, M K and Varshney, R K and Layland, N and Payton, P and Holbrook, C (2017) Next generation sequencing approaches for understanding genetic mechanism of drought tolerance in Valencia Peanut. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.