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Towards defining heterotic pools for accelerating hybrid breeding in pigeonpea

OAR@ICRISAT

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Relation http://oar.icrisat.org/10314/
 
Title Towards defining heterotic pools for accelerating hybrid breeding in pigeonpea
 
Creator Saxena, R K
Singh, V
Rathore, A
Yu, O
Kim, C
Kumar, V
Patel, K
Khan, A W
Tyagi, K
An, S
Seon, D
Kuruvinashetti, M S
Kim, J
BellbullKim, -
Zhang, W
Sameer Kumar, C V
Muniswamy, S
Saxena, K B
Reif, J C
Varshney, R K
 
Subject Pigeonpea
Genetics and Genomics
 
Description Recently released pigeonpea hybrids for cultivation in farmers’
fields have shown their potential to elevate the yield levels. For
instance, the world’s first grain legume hybrid of pigeonpea
ICPH 2671 showed 47% yield advantage over the check varieties.
Generally, the development of such hybrids is based on
selection efficiency of breeding program; breeders make thousands
of random crosses between cytoplasmic male sterile
(CMS) lines and tester lines. In order to enhance the selection
efficiency, genomic diversity along with the phenotyping data
have been used for predicting the best possible parental combinations.
This approach has been successfully used in defining
heterotic pools in many crop species such as maize, rice, sunflower,
and rapeseeds.. With an aim to define heterotic pools in
pigeonpea, a set of 104 parental lines (09 CMS, 13 maintainers
and 82 restorers) have been re-sequenced following whole genome
re-sequencing (WGRS) approach. WGRS yielded a total
of 511 GB data with the coverage ranging from 5X to 10X. A
total of 3.4 million SNPs have been identified across parental
lines. Structural variations such as copy number and presence/
absence variations have been also identified. In parallel, these
parental lines have been used to develop test crosses in factorial
mating design. F1 hybrids along with parental lines were
phenotyped for yield and yield related traits at two locations
in India, namely ICRISAT, Telangana State and ARS-Gulbarga,
Karnataka. The availability of genome-wide SNP variations
combined with the phenotypic data will be used for deploying
genomic selection to define hetereotic pools in pigeonpea for
accelerating hybrid breeding program.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/10314/1/Abstract_Book_420.pdf
Saxena, R K and Singh, V and Rathore, A and Yu, O and Kim, C and Kumar, V and Patel, K and Khan, A W and Tyagi, K and An, S and Seon, D and Kuruvinashetti, M S and Kim, J and BellbullKim, - and Zhang, W and Sameer Kumar, C V and Muniswamy, S and Saxena, K B and Reif, J C and Varshney, R K (2017) Towards defining heterotic pools for accelerating hybrid breeding in pigeonpea. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.