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Haplotype for drought responsive genes in pigeonpea

OAR@ICRISAT

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Relation http://oar.icrisat.org/10316/
 
Title Haplotype for drought responsive genes in pigeonpea
 
Creator Sinha, P
Saxena, R K
Singh, V K
Khan, A W
Upadhyaya, H D
Desai, A
Sameer Kumar, C V
Varshney, R K
 
Subject Abiotic Stress
Drought Tolerance
Pigeonpea
Drought
Genetics and Genomics
 
Description Pigeonpea is a resilient crop which is relatively drought tolerant
compared to many other legumes. To understand the
molecular mechanisms of this unique feature in pigeonpea, 51
genes were selected using the Hidden Markov Models (HMM).
These genes were validated for their expression on parents of
mapping populations (ICPL 151, ICPL 8755, and ICPL 227) segregating
for drought tolerance. A total of 10 differentially expressed
genes that showed ≥2-fold up-regulation in the higher
drought tolerant genotypes was identified. Further analysis of
the 10 genes revealed that they encode four different classes of
proteins. These include plant U-box protein (four genes), universal
stress protein A-like protein (four genes), cation/H(+)
antiporter protein (one gene) and an uncharacterized protein
(one gene). Subsequently, these 10 candidate genes were used
for haplotype analysis in whole genome re-sequencing data of
292 lines. As a result, 10 to 56 haplotypes were identified for
the selected 10 genes. In order to validate the haplotype results,
a defined set of lines is being phenotyped for induced
water stress using polyethylene glycol (PEG) at seedling stage
along with the non-stressed control seedlings. Phenotyping
data together with the haplotype information of the targeted
candidate genes will be useful for the identification of superior
haplotypes of genes. The lines possessing superior haplotypes
of the candidate genes could be used in genomics-assisted
breeding programs for development of more drought tolerant
lines.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/10316/1/Abstract_Book_425a.pdf
Sinha, P and Saxena, R K and Singh, V K and Khan, A W and Upadhyaya, H D and Desai, A and Sameer Kumar, C V and Varshney, R K (2017) Haplotype for drought responsive genes in pigeonpea. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.