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Characterization of groundnut resistance to bacterial-wilt caused by Ralstonia solanacearum by forward and reverse genetics methods

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Relation http://oar.icrisat.org/10318/
 
Title Characterization of groundnut resistance to bacterial-wilt caused by Ralstonia solanacearum by forward and reverse genetics methods
 
Creator Zhang, C
Zhang, N
Zhuang, R R
Chen, H
Cai, T C
Gandeka, M
Niaz, A
He, G H
Rashney, R K
Zhuang, W J
 
Subject Groundnut
Genetics and Genomics
Plant Disease
 
Description Bacterial wilt caused by casual agent Ralstonia solanacearum (Rs)
is a serious disease in groundnut and great many other plant species.
Forward and reverse genetics strategies were adopted in
our study. From the view point of forward genetics, the peanut
RILs including 300 F9 progenies derived from Yueyou 92 × Xinhuixiaoli
were tested of disease resistance through inoculation
with R.solanacearum in the field. Resistance to R.solanacearum
is a quantitative trait. Three QTLs were mapped on an interval
15 cM using a linkage map with SSR and related SNP markers.
An Tir-NBS-LRR resistance gene AhqBW3 was found closely
linked with a BW resistance molecular marker SNP79 which was
mapped in a gene locus next to the R gene. AhqBW3 was showed
downregulation under the challenge of Rs inoculation. From the
view point of reverse genetics, a novel NBS-LRR resistance gene
AhRRS5, an LRR-RLK gene AhRLK1 and an unkown gene AhRRS22
were upregulated by Rs inoculation which were screened from
microarray hybridization. They showed resistance phenotypes in
transgenic tobacco overexpressing of these three genes. Among
of them, Overexpression of AhRRS5 significantly enhanced the
resistance of heterogeneous tobacco to R. solanacearum, with
diverse resistance levels in different transgenic lines. Several
defense-responsive marker genes in hypersensitive response,
including HR, SA, JA, and ET signals, were considerably upregulated
in the transgenic lines as compared with the wild type in response
to R. solanacearum. NPR1 and NDR1 were also upregulated
in response to the pathogen. These results indicate that AhRRS5
participates in the defense response to R. solanacearum through
the crosstalk of multiple signaling pathways and the involvement
of NPR1 and R gene signals for its resistance. These studies may
guide the resistance enhancement of peanut and other economic
crops to bacterial wilt disease.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10318/1/Abstract_Book_431.pdf
Zhang, C and Zhang, N and Zhuang, R R and Chen, H and Cai, T C and Gandeka, M and Niaz, A and He, G H and Rashney, R K and Zhuang, W J (2017) Characterization of groundnut resistance to bacterial-wilt caused by Ralstonia solanacearum by forward and reverse genetics methods. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.