Record Details

Fine mapping of stay-green QTLs on sorghum chromosome SBI-10L ‒ An approach from genome to phenome

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/10319/
 
Title Fine mapping of stay-green QTLs on sorghum chromosome SBI-10L ‒ An approach from genome to phenome
 
Creator Usha Kiranmayee, K N S
Hash, C T
Kavi Kishor, P B
Ramu, P
Sivasubramani, S
Sakhale, S A
Deshpande, S P
 
Subject Sorghum
Genetics and Genomics
 
Description Sorghum is the fifth most important C4 cereal crop grown
globally in arid and semi-arid climatic conditions. Drought is
the major cause for loss of productivity worldwide. Delayed
senescence of plants leads to adaptation to drought stress
conditions by staying-green and giving high yields. In order
to identify and dissect the stay-green genomic regions we
have developed a high resolution fine mapping population
from introgression line cross RSG04008-6 (stay-green) ×
J2614-11 (shoot fly resistant). Nearly 1894 F2 genotypes were
screened with 8 SSR in order to identify double recombinants
for both the parents in the sorghum chromosome-10 long arm
(SBI-10L). The selected F2:4 recombinants were GBSed to increase
the marker density between flanking markers Xgap001-
Xtxp141 for stay-green QTLs on SBI-10L and a high resolution
linkage map was developed using GBS SNPs. Out of 182 only
152 recombinants were replicated thrice in field for staygreen
screening for two seasons (Summer 2013 and 2014).
Fine mapping of the per cent Green Leaf Area (%GLA) traits
identified 33 QTLs and 19QTLs were clustered into 7groups
where 8 genes were identified. These were AP2/ERF transcription
factor family (Sb10g025053), Ankyrin-repeat protein
(Sb10g025310), WD40 repeat protein (Sb10g025320), NBSLRR
Protein (Sb10g025283), Calcium dependant protein kinase
(Sb10g030150), LEA2 protein (Sb10g029570), a putative
uncharacterized protein (Sb10g024920) and senescence associated
protein (Sb10g030520). With this study SBI-10L staygreen
genomic regions were delimited from 15Mb to 8 genes
co-localized with GWAS MTAs. Further cloning and expression
level studies of the identified candidate genes will improve the
development of drought tolerant genotypes.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/10319/1/Abstract_Book_Final_427.pdf
Usha Kiranmayee, K N S and Hash, C T and Kavi Kishor, P B and Ramu, P and Sivasubramani, S and Sakhale, S A and Deshpande, S P (2017) Fine mapping of stay-green QTLs on sorghum chromosome SBI-10L ‒ An approach from genome to phenome. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.