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Multiplex PCR assay for the detection of aflatoxigenic and non-aflatoxigenic Aspergilli.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/13171/
http://dx.doi.org/10.3923/jm.2008.136.142
 
Title Multiplex PCR assay for the detection of aflatoxigenic and non-aflatoxigenic Aspergilli.
 
Creator Latha, R.
Manonmani, H. K.
Rati, E. R.
 
Subject 09 Food Microbiology
 
Description Aflatoxins are potent secondary metabolites produced commonly by Aspergillus flavus, A. parasiticus and A. nomius. Primers were designed specifically for o-methyl transferase (omt) and aflatoxin regulatory gene (aflR) of aflatoxin biosynthetic pathway and also to detect the genus Aspergillus specific primers (18s rRNA genes) using NS. Experimental conditions were standardized for optimum multiplex PCR. DNA extracted from mycelia of toxigenic and non-toxigenic A. flavus, A parasiticus, other Aspergilli and from other genera of fungi were subjected to multiplex PCR using these primers. The omt and aflR primer pairs gave specific PCR amplification for aflatoxigenic A. flavus and A. parasiticus. They did not give DNA amplification for non-aflatoxigenic A. flavus, A. oryzae, A. glaucus, Fusarium, Penicillium and Rhizopus spp.

Multiplex PCR assay for the detection of aflatoxigenic and non-aflatoxigenic Aspergilli. Available from:
 
Date 2008
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13171/1/136-142.pdf
Latha, R. and Manonmani, H. K. and Rati, E. R. (2008) Multiplex PCR assay for the detection of aflatoxigenic and non-aflatoxigenic Aspergilli. Research Journal of Microbiology, 3 (3). pp. 136-142.