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Studies on Molecular Targets and Pathways Regulated by Rice RFL for Flowering Transition and Panicle Development

Electronic Theses of Indian Institute of Science

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Title Studies on Molecular Targets and Pathways Regulated by Rice RFL for Flowering Transition and Panicle Development
 
Creator Goel, Shipra
 
Subject Rice RFL
Flowering Transition
Rice Inflorescences
Axillary Meristem
Rice Floral Development
Rice Panicle Development
Arabidopsis thaliana LFY
RFL-Rice FLO-LFY Homolog
Genetc Transcription Regulation
Inflorescence Meristem
Vegetative Axillary Meristems
Cell Biology
 
Description LFY of Arabidopsis is a member of a unique plant specific transcription factor family. It is involved in giving meristem a determinate floral fate by the activation of floral organ identity genes and preventing inflorescence meristem identity. RFL is a homolog of FLO/LFY in rice. Studies from our lab on rice RFL, based on the effects of knockdown or overexpression, showed its major functions are in timing the conversion of SAM to IM and to prevent the premature conversion of branch meristem to spikelets. Additionally roles in vegetative axillary meristem specification have been also been identified in laboratory. Here, we attempt to delineate molecular pathways directly regulated by RFL as a transcription factor controlling inflorescence and floral development in rice.
Part I: Identification of global target genes bound by RFL in developing rice inflorescences
We carried out ChIP sequencing of the DNA bound by RFL in panicles (01.-0.3cm stage) using anti-RFL antibody. DNA sequences in one library pool were analyses by the MACS algorithm (FDR
 
Contributor Vijayaraghavan, Usha
 
Date 2017-11-28T16:20:47Z
2017-11-28T16:20:47Z
2017-11-28
2016
 
Type Thesis
 
Identifier http://hdl.handle.net/2005/2826
http://etd.ncsi.iisc.ernet.in/abstracts/3676/G27879-Abs.pdf
 
Language en_US
 
Relation G27879