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Isolation of anti-listerial bacteriocin producing Lactococcus lactis CFR-B3 from Beans (Phaseolus vulgaris).

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Title Isolation of anti-listerial bacteriocin producing
Lactococcus lactis CFR-B3 from Beans (Phaseolus
vulgaris).
 
Creator Kiran Kumar, P.
Badarinath, V.
Prakash, M. Halami
 
Subject 10 Food Microorganisms
22 Legumes-Pulses
 
Description Contamination of food samples by Listeria
monocytogenes is a major cause of food-borne infections. Controlling the growth of Listeria sp. by application of bacteriocin or
bacteriocin producing-organism in foods is one of the challenges in the development of effective bio-preservation process. The
objective of this study was to isolate and to characterize bacteriocinogenic strain Lactococcus lactis CFR-B3 from Indian beans.
Bacterial strain of Pediococcus acidilactici NRRL B1153 was used as indicator to screen bacteriocin production. The culture was
found to produce class IIa heat-stable anti-listerial bacteriocin of MW ~ 5 kDa. The use of food-grade organism as an indicator
for screening bacteriocinogenic strains of lactic acid bacteria circumvent the need for using pathogenic cultures such as listeria
spp. The native isolate Lc. lactis CFR-B3 finds its application in the preservation of vegetables due to its prevalence in the same
ecological niche.
 
Date 2008
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13211/1/The%20Internet%20Journal%20of%20Microbiology.pdf
Kiran Kumar, P. and Badarinath, V. and Prakash, M. Halami (2008) Isolation of anti-listerial bacteriocin producing Lactococcus lactis CFR-B3 from Beans (Phaseolus vulgaris). The Internet Journal of Microbiology, 6 (1). pp. 1-6.