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Molecular Analyses of Vanilla planifolia Cultivated in India using RAPD and ISSR Markers.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/13236/
 
Title Molecular Analyses of Vanilla planifolia
Cultivated in India using RAPD and ISSR Markers.
 
Creator Sreedhar, R. V.
Venkatachalam, L.
Roohie, K.
Bhagyalakshmi, N.
 
Subject 10 Plants
03 Biochemistry & Molecular Biology
 
Description The natural “vanilla flavour” obtained from the pods of Vanilla planifolia is the largest flavouring ingredient in food industry. In order to
evaluate the usefulness of genetic markers such as RAPD and ISSR for assessing the diversity among clones of V. planifolia and to create
a database for the available germplasm, we investigated the existing population cultivated in India. The genetic diversity among 25
accessions collected from 13 major locations was studied. Forty random amplified polymorphic DNA (RAPD) and 11 inter-simple
sequence repeats (ISSR) primers resulted in 326 scorable bands ranging in size from 200 bp to 2800 bp and 83 scorable bands from 200
bp to 2500 bp, respectively. Banding pattern among the different samples collected within accessions was similar indicating that the
morphological difference observed within accession had no genetic background. On the other hand, molecular analysis among different
accessions from different locations also yielded identical PCR band profiles in both RAPD and ISSR analysis. These results clearly
indicate that V. planifolia cultivated in India is likely to share the same genetic background and therefore, the genetic diversity is
extremely low. Therefore we propose other biotechnological approaches to induce genetic variations to improve the agronomical traits.
 
Date 2007
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13236/1/Orchid%20Science%20and%20Biotechnology%201%281%29%2C%2029-33%202007.pdf
Sreedhar, R. V. and Venkatachalam, L. and Roohie, K. and Bhagyalakshmi, N. (2007) Molecular Analyses of Vanilla planifolia Cultivated in India using RAPD and ISSR Markers. Orchid Science and Biotechnology, 1 (1). pp. 29-33.