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Epigallocatechin gallate protects BEAS-2B cells from lipopolysaccharide-induced apoptosis through upregulation of gastrin-releasing peptide.

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Relation http://ir.cftri.com/13237/
http://dx.doi.org/10.1007/s11010-017-3040-y
 
Title Epigallocatechin gallate protects BEAS-2B cells
from lipopolysaccharide-induced apoptosis through upregulation
of gastrin-releasing peptide.
 
Creator Divya, P.
Bijesh, Puthusseri
Denny Joseph, K. M.
Savanur, M. A.
 
Subject 32 Antioxidants
 
Description Gastrin-releasing peptide (GRP) plays a major
role in the development and maintenance of lung epithelial
cells by promoting cell division, whereas its suppression
causes growth arrest and apoptosis. The present study
shows that human bronchial epithelial BEAS-2B cells
challenged with lipopolysaccharide (LPS), an endotoxin
from gram-negative bacteria, downregulated GRP expression
and induced apoptosis via upregulation of p53 and
active caspase-3, signifying the importance of GRP in lung
epithelial cell survival. However, in the presence of epigallocatechin-
3-gallate (EGCG), a polyphenol in green tea,
BEAS-2B cells resisted LPS-induced apoptosis and
restored the expression of GRP and its downstream effectors
such as epidermal growth factor receptor and NF-jB,
as analysed by immunoblotting and qPCR. Based on our
findings, we objectify that cytoprotective functions of
EGCG, via upregulation of GRP in cells challenged with
LPS, are novel and can be further explored in a therapeutic
point of view for diseases such as septic shock.
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13237/1/Mol%20Cell%20Biochem%20%282017%29%20434105%E2%80%93111.pdf
Divya, P. and Bijesh, Puthusseri and Denny Joseph, K. M. and Savanur, M. A. (2017) Epigallocatechin gallate protects BEAS-2B cells from lipopolysaccharide-induced apoptosis through upregulation of gastrin-releasing peptide. Molecular and Cellular Biochemistry, 434. pp. 105-111. ISSN 0300-8177