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C-Glycosylated flavonoids from black gram husk: Protection againstDNA and erythrocytes from oxidative damage and their cytotoxiceffect on HeLa cells.

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Relation http://ir.cftri.com/13260/
http://dx.doi.org/10.1016/j.toxrep.2016.08.006
 
Title C-Glycosylated flavonoids from black gram husk: Protection againstDNA and erythrocytes from oxidative damage and their cytotoxiceffect on HeLa cells.
 
Creator Girish, T. K.
Anil Kumar, K.
Prasada Rao, U. J. S.
 
Subject 18 Flavonoid Chemistry
07 Waste utilization
22 Legumes-Pulses
 
Description tC-Glycosyl flavones are present in different plant tissues and they exhibit health benefits. In the presentstudy, it was found that C-glycosyl flavones are distributed in different milled fractions of black gram andamong these fractions, husk had the highest content of C-glycosyl flavones. Two C-glycosyl flavones fromblack gram husk were extracted and purified by preparative high-performance liquid chromatography(HPLC) column. The purity of each compound was assessed by analytical C18 column. The structure of eachcompound was confirmed by LC–MS/MS, NMR. The molecular mass of these compounds were found to be[M−H]−, m/z 431.36 and [M−H]−, m/z 431.35 and were identified as vitexin and isovitexin, respectively.Content of vitexin and isovitexin in aqueous ethanol extract was found to be 76 and 65 mg/g of extract,respectively. These C-glycosyl flavones protected DNA and erythrocytes from oxidative damage. The IC50values for vitexin, isovitexin and quercetin for hemolysis were 6, 5.7 and 2.37 �g, respectively. Thesecompounds also triggered the process of apoptosis in HeLa cells by downregulating Bcl-2 level with thesimultaneous upregulation of Bax and caspase-3 protein expression. Thus, C-glycosyl flavones from blackgram husk protected DNA and erythrocytes from oxidative damage and exhibited anticancer activity.
 
Date 2016
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13260/1/Toxicology%20Reports%203%20%282016%29%20652%E2%80%93663.pdf
Girish, T. K. and Anil Kumar, K. and Prasada Rao, U. J. S. (2016) C-Glycosylated flavonoids from black gram husk: Protection againstDNA and erythrocytes from oxidative damage and their cytotoxiceffect on HeLa cells. Toxicology Reports, 3. pp. 652-663.