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Immobilization of carbonic anhydrase enriched microorganism on biopolymer based materials

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Title Immobilization of carbonic anhydrase enriched microorganism on
biopolymer based materials
 
Creator Prabhu, Chandan
Wanjari, Snehal
Gawande, Sonia
Das, Sneha
Labhsetwar, Nitin
Kotwal, Swati
Puri, Adarsh Kumar
Satyanarayana, T
Rayalu, Sadhana
 
Subject Materials Science
 
Description The whole cell of Bacillus pumilus was immobilized on different chitosan based materials while attempts
were also made to immobilize carbonic anhydrase (CA) enzyme. The screening of materials for esterase
activity resulted in the selection of biopolymer based beads as the potential material for whole cell
and CA immobilization. The materials were characterized by Fourier transform infrared (FTIR) spectroscopy,
X-ray diffraction (XRD), scanning electron microscopy (SEM), while physical characteristics
like BET surface area, particle size etc. were also determined. Esterase activity and other parameters, like
effect of swelling, were determined on immobilized cells. After cell immobilization, the esterase activities
of chitosan-NH4OH beads, multilayered beads, and sodium alginate were found to be 42, 36, and
30.5 U/ml, respectively, as compared to 27.15 U/ml for the free organism.
 
Publisher Elsevier
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1010/1/prabhu2009.pdf
Prabhu, Chandan and Wanjari, Snehal and Gawande, Sonia and Das, Sneha and Labhsetwar, Nitin and Kotwal, Swati and Puri, Adarsh Kumar and Satyanarayana, T and Rayalu, Sadhana (2009) Immobilization of carbonic anhydrase enriched microorganism on biopolymer based materials. Journal of Molecular Catalysis B: Enzymatic, 60. pp. 13-21. ISSN 1381-1177
 
Relation https://www.journals.elsevier.com/journal-of-molecular-catalysis-b-enzymatic
http://neeri.csircentral.net/1010/