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Immobilized Carbonic Anhydrase for the Biomimetic Carbonation Reaction

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Title Immobilized Carbonic Anhydrase for the Biomimetic Carbonation Reaction
 
Creator Yadav, Renu
Wanjari, Snehal
Prabhu, Chandan
Kumar, Vivek
Labhsetwar, Nitin
Satyanarayana, T
Kotwal, Swati
Rayalu, Sadhana
 
Subject Materials Science
 
Description Carbonic anhydrase (CA) has been immobilized on surfactant-modified silylated chitosan (SMSC) for the
carbonation reaction. CA immobilized on SMSC was characterized using a scanning electron microscope,
energy-dispersive X-ray and X-ray diffraction spectroscopy, and Fourier transform infrared analysis. The
effect of various parameters, such as pH, temperature, and storage stability, on immobilized CA was
investigated using a p-nitrophenyl acetate (p-NPA) assay. The optimum pH and temperature were
determined to be 7 and 35 �C, respectively. Kinetic parameters of immobilized and free CA (Km and Vmax
values) were also evaluated. For immobilized CA, the Km value was 4.547 mM and the Vmax value was
1.018 mmol min-1mg-1, whereas for the free CA, theKm value was 1.211mMand theVmax value was 1.125
mmol min-1 mg-1. It was observed that immobilized CA had longer storage stability and retained 50% of
its initial activity up to 30 days. Proof of concept has been established for the biomimetic carbonation
reaction. TheCO2 sequestration capacity in terms of conversion ofCO2 to carbonate was quantified by gas
chromatography (GC). It was 10.73 and 14.92 mg of CaCO3/mg of CA for immobilized and free CA,
respectively, under a limiting concentration of CO2 (14.5 mg of CO2/10 mL).
 
Publisher ACS
 
Date 2010-01-11
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1012/1/10.1021%40ef100750y.pdf
Yadav, Renu and Wanjari, Snehal and Prabhu, Chandan and Kumar, Vivek and Labhsetwar, Nitin and Satyanarayana, T and Kotwal, Swati and Rayalu, Sadhana (2010) Immobilized Carbonic Anhydrase for the Biomimetic Carbonation Reaction. Energy & Fuels, 24. pp. 6198-6207. ISSN 0887-0624
 
Relation http://neeri.csircentral.net/1012/