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Effect of arginine : lysine ratio in free amino acid and protein form on L-NAME induced hypertension in hypercholesterolemic Wistar rats.

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Relation http://ir.cftri.com/13274/
http://dx.doi.org/10.1039/c6ra13632j
 
Title Effect of arginine : lysine ratio in free amino acid
and protein form on L-NAME induced hypertension
in hypercholesterolemic Wistar rats.
 
Creator Vishwanath, S. Vallabha
Arun, Tapal
Shinde, V. S.
Govindaraju, K.
Purnima Kaul, Tiku
 
Subject 02 Amino acids
 
Description Arginine plays an important role in cardiovascular diseases, especially as a nitric oxide precursor leading to
vasodilation. The present study focuses, on the effect of the arginine/lysine (Arg : Lys) ratio in the protein
form and free amino acid form on hypertension in hypercholesterolemic Wistar rats. Hypertension was
induced by administration of L-NAME (Nu-nitro-L-arginine methyl ester hydrochloride) orally for 16
weeks in high cholesterol fed Wistar rats. Arginine : lysine ratio is high in Moringa seed protein isolate
(MPI) compared to other oilseed proteins. After inducting, the treatment groups were supplemented with
diets containing Moringa seed protein isolate (MPI-LN) and free amino acids (FAA-LN) for 6 weeks. A
decrease in systolic blood pressure (SBP) was observed after treatment. The MPI-LN and FAA-LN
exhibited 126.6 � 2.2 mmHg and 127.5 � 0.2 mmHg of SBP respectively, which is less compared to the
hypertensive rats (146.7 � 4.5 mmHg). The hypertensive markers such as angiotensin-I converting
enzyme (ACE) and nitrate levels in kidney were analysed. The MPI-LN and FAA-LN exhibited 10.30 � 3.7
nmoles per mg per min and 37.56 � 3.7 nmoles per mg per min ACE activity respectively. In both
treatment groups, a 50% decrease in ACE activity was observed compared to hypertensive rats. Increase
(30%) in nitrate levels was observed in MPI-LN (0.47 � 0.04 mM g�1) and FAA-LN (0.4 � 0.06 mM g�1)
compared to hypertensive rats. From the results, it is evident that Arg : Lys has a role in hypertension
regulation. Lipid profiles of blood plasma showed a decrease in plasma triacylglyceride and liver
triacyglyceride levels in the treatment groups. The Arg : Lys ratio in both the protein form (MPI) and free
amino acid form strongly affects the metabolic pathways of hypertension with moderate effect on
hypercholesterolemia.
 
Date 2016
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13274/1/RSC%20Adv.%2C%202016%2C%206%2C%2073388.pdf
Vishwanath, S. Vallabha and Arun, Tapal and Shinde, V. S. and Govindaraju, K. and Purnima Kaul, Tiku (2016) Effect of arginine : lysine ratio in free amino acid and protein form on L-NAME induced hypertension in hypercholesterolemic Wistar rats. RSC Advances, 6. pp. 73388-73398.