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Carbon composite beads for immobilization of carbonic anhydrase

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Title Carbon composite beads for immobilization of carbonic anhydrase
 
Creator Prabhu, Chandan
Valechha, Aarti
Wanjari, Snehal
Labhsetwar, Nitin
Kotwal, Swati
Satyanarayana, T
Rayalu, Sadhana
 
Subject Materials Science
 
Description Fixation of anthropogenic CO2 into calcium carbonate using carbonic anhydrase (CA) as a bio-catalyst
appears to be a promising option of CO2 sequestration. In the present study, carbonic anhydrase (CA) has
been immobilized on chitosan based activated alumina–carbon composite beads. Synthesized adsorbent
was thoroughly characterized using XRD, FTIR, SEM and BET-SA. Maximum adsorption capacities were
determined using Langmuir model and it was observed to be 172.41 mg/g. Mechanistic and kinetic aspects
have been addressed for immobilized as well as free enzyme. The Km and Vmax for immobilized enzyme
was 10.35mM and 0.99�mol/ml/min, whereas for free enzyme it was 1.89mM and 0.99�mol/ml/min
respectively. Proof of concept has been established for carbonation reaction. The carbonate has been
characterized using various tools such as XRD and SEM which confirms the calcite nature of calcium
carbonate. The CO2 sequestration capacity in terms of conversion of CO2 to carbonate was quantified by
gas chromatography (GC). The CO2 sequestration capacity of immobilized beads was found to be 19.22mg
of CaCO3/mg of enzyme as compared to 33.06mg of CaCO3/mg of enzyme for free enzyme.
 
Publisher Elsevier
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1034/1/prabhu2011.pdf
Prabhu, Chandan and Valechha, Aarti and Wanjari, Snehal and Labhsetwar, Nitin and Kotwal, Swati and Satyanarayana, T and Rayalu, Sadhana (2011) Carbon composite beads for immobilization of carbonic anhydrase. Journal of Molecular Catalysis B: Enzymatic, 71. pp. 71-78. ISSN 1381-1177
 
Relation https://www.journals.elsevier.com/journal-of-molecular-catalysis-b-enzymatic
http://neeri.csircentral.net/1034/