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Immobilization of carbonic anhydrase on chitosan beads for enhanced carbonation reaction

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Title Immobilization of carbonic anhydrase on chitosan beads for enhanced
carbonation reaction
 
Creator Wanjari, Snehal
Prabhu, Chandan
Yadav, Renu
Satyanarayana, T
Labhsetwar, Nitin
Rayalu, Sadhana
 
Subject Materials Science
 
Description Carbonic anhydrase (CA) has been immobilized on chitosan beads and has been tested for its activity
using p-NPA. CA immobilized chitosan beads were further tested for targeted application of carbonation
reaction to convert CO2 to CaCO3. It was observed that onset time for precipitation of CaCO3 was 40 s
for immobilized catalyst as compared to non-catalyzed system (100 s). Mechanistic and kinetic aspects
have been addressed for immobilized catalyst as well as free CA using p-NPA assay. Km and Vmax of the
immobilized CA was observed to be 2.36mM and 0.54�moles/min/ml as compared to Km and Vmax of
0.87mMand 0.93�moles/min, respectively, for free CA. Storage stability test was conducted up to 20 days
and it was observed that at −20 ◦C, the Half Life Period (HLP) of immobilized CA was 216 h as compared
to 192 h for free CA whereas at 25 ◦C the HLP for immobilized and free CA was observed to be 456 h and
408 h, respectively.
 
Publisher Elsevier
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1036/1/wanjari2011.pdf
Wanjari, Snehal and Prabhu, Chandan and Yadav, Renu and Satyanarayana, T and Labhsetwar, Nitin and Rayalu, Sadhana (2011) Immobilization of carbonic anhydrase on chitosan beads for enhanced carbonation reaction. Process Biochemistry, 46. pp. 1010-1018. ISSN 1359-5113
 
Relation https://www.journals.elsevier.com/process-biochemistry
http://neeri.csircentral.net/1036/