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Region-Specific Bacterial Carbonic Anhydrase for Biomimetic Sequestration of Carbon Dioxide

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Title Region-Specific Bacterial Carbonic Anhydrase for Biomimetic
Sequestration of Carbon Dioxide
 
Creator Prabhu, Chandan
Wanjari, Snehal
Puri, Adarsh
Bhattacharya, Abhishek
Pujari, Rajesh
Yadav, Renu
Sera, Sera
Labhsetwar, Nitin
Sharma, Anjana
Satyanarayanan, T
Rayalu, Sadhana
 
Subject Materials Science
 
Description Biomimetic carbonic anhydrase (CA)-based CO2 sequestration for its conversion into carbonate is being pursued all
across the globe. For this purpose, partially purified enzyme CA has been isolated from three different microorganisms, including
Bacillus pumilus, Pseudomonas fragi, and Micrococcus lylae. Proof of concept has been established for the carbonation reaction. The
carbonate has been characterized using various tools, such as X-ray diffraction (XRD) and scanning electron microscopy (SEM),
which confirms the calcite phase of calcium carbonate. The formation of carbonate has been quantified using gas chromatography.
Partially purified CA isolated from P. fragi shows a significantly high CO2 sequestration capacity of 27.33 mg of CaCO3/mg of CA in
15 min compared to B. pumilus and M. lylae. The enzyme has been immobilized on surfactant-modified silylated chitosan. The
immobilized enzyme shows improved storage stability and retained 50% initial activity up to 30 days.
 
Publisher ACS
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/1040/1/10.1021%40ef101608r.pdf
Prabhu, Chandan and Wanjari, Snehal and Puri, Adarsh and Bhattacharya, Abhishek and Pujari, Rajesh and Yadav, Renu and Sera, Sera and Labhsetwar, Nitin and Sharma, Anjana and Satyanarayanan, T and Rayalu, Sadhana (2011) Region-Specific Bacterial Carbonic Anhydrase for Biomimetic Sequestration of Carbon Dioxide. Energy & Fules, 25 (3). pp. 1327-1332. ISSN 0976-5816
 
Relation http://pubs.acs.org/journal/enfuem
http://neeri.csircentral.net/1040/