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Lactucaxanthin – a potential anti-diabetic carotenoid from lettuce (Lactuca sativa) inhibits α-amylase and α-glucosidase activity in vitro and in diabetic rats.

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Relation http://ir.cftri.com/13277/
http://dx.doi.org/10.1039/c6fo01655c
 
Title Lactucaxanthin – a potential anti-diabetic carotenoid from lettuce (Lactuca sativa) inhibits α-amylase and α-glucosidase activity in vitro and in diabetic rats.
 
Creator Sowmyashree, G.
Jhansi Lakshmi, M.
Sharavana, Gurunathan
Gunaseelan, S.
Sreerama, Y. N.
Baskaran, V.
 
Subject 14 Carotenoid Chemistry
04 Diabetes Mellitus
23 Vegetables
 
Description Intestinal and pancreatic α-amylase and α-glucosidase inhibitors offer an approach to lower the levels of post-prandial hyperglycemia through the control of dietary starch breakdown in digestion. This study hypothesized that lactucaxanthin (Lxn) in lettuce (Lactuca sativa) inhibits the activity of α-amylase and α-glucosidase. In this study, the interaction of Lxn with α-amylase and α-glucosidase in silico and its inhibitory effect on these enzymes were studied using in vitro and STZ-induced diabetic rat models. Lxn was isolated from lettuce with 96% purity confirmed by HPLC and LCMS. The in silico analysis showed that Lxn has a lower binding energy (-6.05 and -6.34 kcal mol-1) with α-amylase and α-glucosidase compared to their synthetic inhibitors, acarbose (-0.21 kcal mol-1) and miglitol (-2.78 kcal mol-1), respectively. In vitro α-amylase and α-glucosidase inhibition assays revealed that Lxn had IC50 values of 435.5 μg mL-1 and 1.84 mg mL-1, but acarbose has values of 2.5 and 16.19 μg mL-1. The in vivo results showed an increased activity for α-amylase and α-glucosidase in the intestine (4.7 and 1.30 fold, p < 0.05) and pancreas (1.3 and 1.48 fold, p < 0.05) of STZ induced diabetic rats compared to normal rats. Whereas the activity decreased (p < 0.05) in the Lxn fed diabetic rats, except for the intestinal α-glucosidase activity (1.69 ± 0.12 PNP per min per mg protein). This was confirmed by the low blood glucose level (239.4 ± 18.2 mg dL-1) in diabetic rats fed Lxn compared to the diabetic group (572.2 ± 30.5 mg dL-1, p < 0.05). Lxn significantly inhibited (p < 0.05) the activity of α-amylase and α-glucosidase and could be of medical and nutritional relevance in the treatment of diabetes.
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13277/1/Food%20Funct.%2C%202017.pdf
Sowmyashree, G. and Jhansi Lakshmi, M. and Sharavana, Gurunathan and Gunaseelan, S. and Sreerama, Y. N. and Baskaran, V. (2017) Lactucaxanthin – a potential anti-diabetic carotenoid from lettuce (Lactuca sativa) inhibits α-amylase and α-glucosidase activity in vitro and in diabetic rats. Food and Function, 8 (3). pp. 1124-1131.