Record Details

Lipase Inhibitors from Nigella Sativa and Punica Granatum as an Effective Approach Towards Controlling Obesity.

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/13303/
ttp://dx.doi.org/10.20319/lijhls.2016.22.0119
 
Title Lipase Inhibitors from Nigella Sativa and Punica Granatum as an Effective Approach Towards Controlling Obesity.
 
Creator Shamsiya, T. K.
Manjunatha, J. R.
Manonmani, H. K.
 
Subject 10 Plants
05 Enzymes
24 Fruits
 
Description Two distinct lipase inhibitor cocktails RAYstat4ns and SHAMstat3pg have been purified from seeds of Nigella sativa and Punica granatum respectively. Structural characterization has shown RAYstat4ns to be a mixture of 3 polyunsaturated fatty acids and one saturated fatty acid while SHAMstat3pg is a triglyceride mixture comprising of three different kinds of polyunsaturated fatty acids. Ethanol extracted fractions of seeds of Nigella saliva and Punic a granatum having high lipase inhibitory activity were purified by HPLC followed by TLC to yield respectively RAYstat4ns and SHAMstat3pg . These plant based lipase inhibitors have shown good lipase inhibitory activity against pancreatic lipase with mixed type of inhibition. RAYstat4ns and SHAMstat3pg have also shown inhibition of hormone sensitive lipase. The experimental IC50 of the purified lipase inhibitors RAYstat4ns and SHAMstat3pg was found to be respectively 4.02μg/μl and 7.35 μg/μl for pancreatic lipase and 6.31 μg/μl and 11.45 μg/μl respectively for hormone sensitive lipase. Hence ,these plant-based lipase inhibitors can be used in controlling lipolysis and insulin resistance in addition to inhibiting absorption of dietary lipid into the living system. In vivo studies could help confirm the potency of these plant based isolates as efficient lipase inhibitors.
 
Date 2016
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13303/1/Life%20Vol.%202%20Issue%202%2C%20pp.%2001-19.pdf
Shamsiya, T. K. and Manjunatha, J. R. and Manonmani, H. K. (2016) Lipase Inhibitors from Nigella Sativa and Punica Granatum as an Effective Approach Towards Controlling Obesity. LIFE: International Journal of Health and Life-Sciences, 2 (2). pp. 1-19.