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Discrimination and divergence among Lactobacillus plantarum- group (LPG) isolates with reference to their probiotic functionalities from vegetable origin.

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Relation http://ir.cftri.com/13350/
http://dx.doi.org/doi:10.1016/j.syapm.2016.09.005
 
Title Discrimination and divergence among Lactobacillus
plantarum- group (LPG) isolates with reference to their
probiotic functionalities from vegetable origin.
 
Creator Sundru, Manjulata Devi
Aishwarya, S.
Prakash, M. Halami
 
Subject 02 Bacteriology
23 Vegetables
 
Description The present study was aimed to evaluate the diversity and probiotic properties of Lactobacillus plantarum-group cultures from vegetable origin. First, genotypic diversity of L. plantarum (n=34) was achieved by PCR of Random Amplified Polymorphic DNA and recA gene-specific multiplex PCR. The isolates were segregated into five groups namely, Lactobacillus pentosus, Lactobacillus paraplantarum, Lactobacillus arizonensis, Lactobacillus plantarum subsp. plantarum and argentoratensis. Further discrimination was achieved by restriction fragment length polymorphism of probiotic adhesion genes viz.fbp, mub and msa gene. As determined by nucleotide sequence analysis and bioinformatics Pfam database, the putative Fbp protein had only one FBP domain, whereas Mub protein had 8-10 MUB domain repeats. However, L. pentosus (except CFR MFT9), L. plantarum subsp. argentoratensis (except CFR MFT5) and L. arizonensis (except CFR MFT2) isolates gave no amplicon for the tested marker genes. Selected cultures (n=15) showed tolerance to simulated digestive fluids (20-85%), exhibited auto-aggregation (10-77%), cellular hydrophobicity (12-78%), and broad spectrum of anti-microbial activity. Concurrently, high adherence capacity to mucin was achieved for L. plantarum subsp. plantarum (MCC 2974 and CFR MFT1) and L. paraplantarum (MTCC 9483, MCC 2977, MCC 2978), which had an additional MUB domain repeat.
 
Date 2016
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13350/1/Syst%20Appl%20Microbiol.%202016%20Dec%3B39%288%29562-570.pdf
Sundru, Manjulata Devi and Aishwarya, S. and Prakash, M. Halami (2016) Discrimination and divergence among Lactobacillus plantarum- group (LPG) isolates with reference to their probiotic functionalities from vegetable origin. Systematic and Applied Microbiology, 39 (8). pp. 562-570.