Comparative assessment of physico-chemical characteristics and fibril formation capacity of thermostable carp scales collagen.
IR@CSIR-CFTRI
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Relation |
http://ir.cftri.com/13353/
http://dx.doi.org/10.1016/j.msec.2016.08.047 |
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Title |
Comparative assessment of physico-chemical characteristics and fibril formation capacity of thermostable carp scales collagen. |
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Creator |
Gaurav Kumar, Pal
Suresh, P. V. |
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Subject |
03 Proteins
Fish |
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Description |
Collagen and collagen fibers have been widely documented as a potential and competitive biomaterial for medical applications. However, the searches for safe and realistic new collagen sources are still underway. Currently, fishery by-products (scales), a promising collagen source are usually discarded. In the present study, in vitro fibril-forming ability of the extracted fish scale collagen is reported. The aim of the investigation was to evaluate the concomitant comparison of fibril-forming abilities and characteristics of acid and pepsin soluble collagens fromthe scales of Indianmajor carp catla (Catla catla) and rohu (Labeo rohita). The extracted collagenswere characterized as type I,with a total yield of 2.80–4.11% (w/w). Denaturation temperature determined for all collagens were between 35.9 and 37.7 °C. All collagens exhibited high solubility in acidic pH and low NaCl concentrations. SEMclarified the lyophilized collagens and their fibril-forming capacity. Amino acid content and radical scavenging efficacy were also analyzed for the extracted collagen. The results revealed that extracted scale collagen from a renewable biological source could be used as biomaterials in various sectors. It might be suitable for preparing collagen gel for biomedical devices or as a scaffold for cell culture because of its high stability and fibril formation capacity. |
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Date |
2017
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Type |
Article
PeerReviewed |
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Format |
pdf
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Language |
en
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Identifier |
http://ir.cftri.com/13353/1/Materials%20Science%20and%20Engineering%20C%20Volume%2070%2C%20Part%201%2C%201%20January%202017%2C%20Pages%2032-40.pdf
Gaurav Kumar, Pal and Suresh, P. V. (2017) Comparative assessment of physico-chemical characteristics and fibril formation capacity of thermostable carp scales collagen. Materials Science and Engineering C, 70. pp. 32-40. |
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