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Gold nanoparticles based enzyme biosensor for the detection of chloramphenicol.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/13390/
http://dx.doi.org/10.1016/j.protcy.2017.04.118
 
Title Gold nanoparticles based enzyme biosensor for the detection of
chloramphenicol.
 
Creator Richa, Sharma
Akshath, U. S.
Praveena, Bhatt
Thakur, M. S.
Raghavarao, K. S. M. S.
 
Subject 11 Antibiotics Chemistry
03 Enzyme Biotechnology and Engineering
 
Description Chronic use of chloramphenicol (CAM) antibiotic leads to anaemia and bone marrow suppression resulting in 40 – 50%
mortality. Hence, there is a need to develop an economical, fast and convenient method to detect CAM in milk, honey, shrimp
and other aquaculture products. In the current method, coenzyme A was used to indirectly quantify CAM (since it is the cofactor
product of the acetylation reaction of CAM). Coenzyme A (CoASH) was used to stabilize gold nanoparticles which were
characterized by studying their extinction spectra. The reductant concentration and synthesis time were optimized. With
optimized parameters the proposed system could detect CoASH up to 0.1 nM in buffer, with a linear range of detection from 0.1
μM to 1 mM.
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13390/1/Procedia%20Technology%2027%20%28%202017%20%29%20282%20%E2%80%93%20286.pdf
Richa, Sharma and Akshath, U. S. and Praveena, Bhatt and Thakur, M. S. and Raghavarao, K. S. M. S. (2017) Gold nanoparticles based enzyme biosensor for the detection of chloramphenicol. Procedia Technology, 27. 282 -286.