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Whey protein isolate enrichment attenuates manganese-induced oxidative stress and neurotoxicity in Drosophila melanogaster: Relevance to Parkinson’s disease.

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Relation http://ir.cftri.com/13405/
http://dx.doi.org/10.1016/j.biopha.2017.09.099
 
Title Whey protein isolate enrichment attenuates manganese-induced oxidative
stress and neurotoxicity in Drosophila melanogaster: Relevance to Parkinson’s
disease.
 
Creator Greeshma, M.
Sriranjini, Venkata Rao
Dr., Muralidhara
Rajini, P. S.
 
Subject 03 Proteins
07 Waste utilization
27 Dairy products
 
Description Currently, much emphasis is being laid on understanding the role of nutrition to alleviate neurodegenerative
conditions. Here we employed a Drosophila model to test the hypothesis that Whey protein isolate (WPI) enrichment
can attenuate Manganese (Mn) induced locomotor phenotype, oxidative stress and neurotoxicity. Mn
exposure (0–30 mM) to young (8–10 d old) males/females and aged (30–35 d old) males elicited a concentration-
dependent temporal mortality and locomotor deficits. In general, females and aged males were more susceptible
than young males. Mn exposure (5–15 mM, 5 d) among male flies, besides induction of oxidative stress
(elevated levels of MDA and protein carbonyls with a concomitant decrease in glutathione levels), caused significant
perturbations in the activities of antioxidant enzymes and mitochondrial complexes. In the co-exposure
paradigm, WPI fortification (0.25–0.5%) markedly ameliorated Mn-induced locomotor phenotype, mortality,
oxidative stress and restored the activity of the antioxidant enzymes, cholinergic function, and dopamine levels.
In a satellite study, flies given prophylactic treatment (10 d) with WPI were more resistant(evidenced by a lower
incidence of mortality and locomotor deficits) to an acute MPP+ challenge suggesting its potential to enhance
survival pathways. Collectively, these data suggest the potential of WPI enrichment to attenuate Mn-induced
toxic manifestations in the Drosophila model. Based on these findings, we propose that fortification of diet with
WPI offers a useful approach to alleviate Mn-induced toxic manifestations and neuronal dysfunctions in humans,
especially in occupational settings.
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13405/1/Biomedicine%20%26%20Pharmacotherapy%2095%20%282017%29%201596%E2%80%931606.pdf
Greeshma, M. and Sriranjini, Venkata Rao and Dr., Muralidhara and Rajini, P. S. (2017) Whey protein isolate enrichment attenuates manganese-induced oxidative stress and neurotoxicity in Drosophila melanogaster: Relevance to Parkinson’s disease. Biomedicine & Pharmacotherapy, 95. pp. 1596-1606.