Record Details

Reactivation of immobilized acetyl cholinesterase in an amperometric biosensor for organophosphorus pesticide.

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/2107/
 
Title Reactivation of immobilized acetyl cholinesterase in an amperometric biosensor for organophosphorus pesticide.
 
Creator Gulla, K. C.
Gouda, M. D.
Thakur, M. S.
Karanth, N. G.
 
Subject 26 Pesticide Chemistry
05 Enzymes
 
Description Biosensors based on acetyl cholinesterase (AChE) inhibition have been known for monitoring of pesticides in food and water samples. However, strong inhibition of the enzyme is a major drawback in practical application of the biosensor which can be overcome by reactivation of the enzyme for repeated use. In the present study, enzyme reactivation by oximes was explored for this purpose. Two oximes viz., 1,1'-trimethylene bis 4-formylpyridinium bromide dioxime (TMB-4) and pyridine 2-aldoxime methiodide (2-PAM) were compared for the reactivation of the immobilized AChE. TMB-4 was found to be a more efficient reactivator under repeated use, retaining more than 60% of initial activity after 11 reuses, whereas in the case of 2-PAM, the activity retention dropped to less than 50% after only 6 reuses. Investigations also showed that reactivation must be effected within 10 min after each analysis to eliminate the ageing effect, which reduces the efficiency of reactivation.
 
Date 2002
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/2107/1/Biochimica%20et%20Biophysica%20Acta%201597%20%282002%29%20133%E2%80%93%20139.pdf
Gulla, K. C. and Gouda, M. D. and Thakur, M. S. and Karanth, N. G. (2002) Reactivation of immobilized acetyl cholinesterase in an amperometric biosensor for organophosphorus pesticide. Biochimica et Biophysica Acta, 1597 (1). pp. 133-9. ISSN 0006-3002