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Novel Folate Binding Protein in Arabidopsis Expressed during Salicylic Acid-Induced Folate Accumulation.

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Relation http://ir.cftri.com/2384/
http://dx.doi.org/10.1021/acs.jafc.7b04236
 
Title Novel Folate Binding Protein in Arabidopsis Expressed during
Salicylic Acid-Induced Folate Accumulation.
 
Creator Bijesh, Puthusseri
Divya, P.
Veeresh, Lokesh
Gyanendra, Kumar
 
Subject 10 Plants
04 Vitamins
 
Description Increasing the quantity of natural folates in plant foods is recently gaining significant interest, owing to their
acute deficiencies in various populations. This study observed that foliar salicylic acid treatment enhanced the accumulation of
folates in Arabidopsis, which correlated with the increase in a folate binding protein (FBP) and the expression of mRNA of a
putative folate binding protein At5G27830. A protein band corresponding to ∼43 kDa was observed after resolving the affinitypurified
protein on SDS-PAGE, and the partial amino acid sequence indicated that the protein is indeed At5G27830. Docking
studies performed with At5G27830 confirmed specific binding of folic acid to predicted site. Heterologous expression of
At5G27830 in the yeast resulted in significant uptake and accumulation of folic acid in cells. This novel study of a plant FBP will
be useful for folate metabolic engineering of a wide range of crops.
 
Date 2018
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/2384/1/J.%20Agric.%20Food%20Chem.%202018%2C%2066%2C%20505%E2%88%92511.pdf
Bijesh, Puthusseri and Divya, P. and Veeresh, Lokesh and Gyanendra, Kumar (2018) Novel Folate Binding Protein in Arabidopsis Expressed during Salicylic Acid-Induced Folate Accumulation. Journal of Agricutural and Food Chemistry, 66. pp. 505-511.