ICAR Research Data Repository for Knowledge Management
Isolation, purification, and some of the properties of hemi-cellulase from fusarium sp.
IR@CSIR-CFTRI
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| Relation |
http://ir.cftri.com/10810/
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| Title |
Isolation, purification, and some of the properties of hemi-cellulase from fusarium sp.
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| Creator |
Dharmaraj, B. Wankhede
Vijayalakshmi, K. R. Madhava Rao, R. Raghavendra, Rao |
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| Subject |
04 Fungi
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| Description |
An extracellular hemicellulase from a soil fungus (Fusarium sp.) grown on a medium containing groundnut hemicellulose B was purified 76-fold by ammonium sulphate fractionation, chromatography on DEAE-cellulose, and gel filtration on Sephadex G-100. It was found to be homogeneous by disc-gel electrophoresis at pH 8. It showed optimal activity at pH 5.6 and 37°. It was observed that groundnut and sesame hemicellulose B were degraded considerably (~80 and 58%, respectively) by the purified hemicellulase, whereas glucomannan and xylan from groundnut were comparatively poorly hydrolysed (~30–40%).
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| Date |
1981
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/10810/1/Carbohydrate%20Research%2C%2096%20%281981%29%20249-257.pdf
Dharmaraj, B. Wankhede and Vijayalakshmi, K. R. and Madhava Rao, R. and Raghavendra, Rao (1981) Isolation, purification, and some of the properties of hemi-cellulase from fusarium sp. Carbohydrate Research, 96 (2). pp. 249-257. |
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