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Effects of n-3 polyunsaturated fatty acids on the reacylation of arachidonic acid in peritoneal macrophages

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Relation http://ir.cftri.com/10816/
 
Title Effects of n-3 polyunsaturated fatty acids on the reacylation of arachidonic acid in peritoneal macrophages
 
Creator Lokesh, B. R.
Kinsella, J. E.
 
Subject 17 Fatty Acid Chemistry
 
Description Mouse peritoneal macrophages were prelabelled with [3H]AA and activated ex vivo with phorbol myristate acetate (PMA) in the presence or absence of added n-3 polyunsaturated fatty acids (n-3 PUFA). The n-3 PUFA did not affect the release of [3H]AA from stimulated macrophages. Added AA, enhanced, in a dose-dependent manner, the amount of free [3H]AA in the medium after activating the cells with PMA. Both n-3 PUFA and AA were actively acylated into the lipids of PMA activated macrophages, but the n-3 PUFA did not compete with reacylation of AA. In unstimulated cells, in the absence of PMA, n-3 PUFA effectively competed with AA for acylation into membrane phospholipids. These studies suggest that distinct pools of AA exist in macrophages.
 
Date 1994
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/10816/1/Ftostaglandina%20Leukomenes%20and%20Essential%20Fatty%20Acids%20C19%2094%29%205%201.%20235-239.pdf
Lokesh, B. R. and Kinsella, J. E. (1994) Effects of n-3 polyunsaturated fatty acids on the reacylation of arachidonic acid in peritoneal macrophages. Prostaglandins, Leukotrienes and Essential Fatty Acids, 51 (4). pp. 235-239. ISSN 0952-3278