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Supercritical carbon dioxide extraction of xanthones with antioxidant activity from Garcinia mangostana: Characterization by HPLC/LC-ESI-MS

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Relation http://ir.cftri.com/9087/
http://www.sciencedirect.com/science/article/B6VMF-4VXDTY3-1/2/cef2da9abc94ef76014e923b04bb8d98
http://dx.doi.org/10.1016/j.supflu.2009.03.004
 
Title Supercritical carbon dioxide extraction of xanthones with antioxidant activity from Garcinia mangostana: Characterization by HPLC/LC-ESI-MS
 
Creator Zarena, A. S.
Udaya Sankar, K.
 
Subject 06 Chromatography
24 Fruits
32 Antioxidants
 
Description The xanthones from mangosteen (Garcinia mangostana) pericarp was extracted with and without alcohol as entrainer at 30MPa and 50C in supercritical carbon dioxide (SC-CO2). It was found that entrainer increases the rate of extraction and yield substantially. SC-CO2+ethanol (4%) at 20MPa, 40C was investigated by HPLC/LC-ESI-MS for characterization of the xanthones. From liquid chromatography electron spray ionization mass spectrometry (LC-ESI-MS) spectra, 12 components were structurally correlated and confirmed based on the fragmentation characteristics and information available in the literature. The occurrence of these xanthone in SC-CO2 extract is reported for first time in G. mangostana species. The antioxidant activities of SC-CO2 were investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals (IC50=41.8[mu]g/mL), lipid peroxidation (IC50=21.5[mu]g/mL), hydroxyl radicals site specific (IC50=30.0[mu]g/mL) and hydroxyl radicals non-site specific (IC50=23.1[mu]g/mL). From cytochrome c assay it was observed that SC-CO2 extract can reduce ferric cytochrome c to ferrocytochrome c. The results showed that SC-CO2 had great potential as antioxidant agent.
 
Date 2009
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/9087/1/J.%20of%20Supercritical%20Fluids%2049%20%282009%29%20330%E2%80%93337.pdf
Zarena, A. S. and Udaya Sankar, K. (2009) Supercritical carbon dioxide extraction of xanthones with antioxidant activity from Garcinia mangostana: Characterization by HPLC/LC-ESI-MS. The Journal of Supercritical Fluids, 49 (3). pp. 330-337. ISSN 0896-8446