Record Details

Purification and characterization of lipase from rice (Oryza sativa L.) bran.

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/7843/
 
Title Purification and characterization of lipase from rice (Oryza sativa L.) bran.
 
Creator Rajeshwara, A. N.
Prakash, V.
 
Subject 05 Enzymes
01 Rice
 
Description Lipase was isolated from defatted rice bran by a new procedure which yields higher enzyme activity and quantity of enzyme. The procedure involves selective ammoniuum sulphate precipitation followed by ion exchange chromatography using DEAE-Sepharose CL-6B gel and column chromatography. The purified and homogeneous enzyme was characterized for optical, physicochemical and hydrodynamic properties. The enzyme had a UV absorption max. at 276 nm with an extinction coeff. value of 15.25. Fluorescence excitation and emission max. were 285 and 344 nm, respectively. Specific activity was 18 units, and the enzyme had a pH optimum of 7.5 and temp. optimum of 30
 
Date 1995
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/7843/1/Die%20Nahrung%2039%20%281995%29%2056%2C%20406-418.pdf
Rajeshwara, A. N. and Prakash, V. (1995) Purification and characterization of lipase from rice (Oryza sativa L.) bran. Nahrung, 39 (5/6). 406-418, 22 ref..