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Expression Analysis of Novel microRNAs in Rice During High Temperature Stress

OAR@ICRISAT

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Relation http://oar.icrisat.org/10463/
http://www.bepls.com/spl_2017(1)/46.pdf
 
Title Expression Analysis of Novel microRNAs in Rice During High
Temperature Stress
 
Creator Sailaja, B
Mangrauthia, S K
Voleti, S R
Subrahmanyam, D
Brajendra, -
Ravindra Babu, V
 
Subject Abiotic Stress
Rice
Genetics and Genomics
Climate Change
 
Description MicroRNAs (miRNAs) are small non-coding RNAs which play an important role in regulating the genes involved in plant
growth and development. Several studies showed that miRNAs are involved in plants response to different kinds of
abiotic stresses also. In our previous study, temperature responsive miRNAs were predicted in O.sativa. 27 miRNAs were
predicted to be novel in rice using homology search. In continuation to our previous study, expression of 14 novel
miRNAs was done in shoot and root of 13 days old seedlings of five different rice cultivars using real time PCR. Expression
these miRNAs was analyzed in control and high temperature stress environment. Out of 14 predicted novel miRNAs, two
novel miRNAs- miR157a and miR165a showed expression in all five rice cultivars. Interestingly, miR165a showed a
differential expression pattern among heat tolerant (N22, IR64 and Rasi) and susceptible (Vandana and Sampada)
cultivars suggesting that it might have specific role in high temperature tolerance.
 
Publisher Academy for Environment and Life Sciences, India
 
Date 2017
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/10463/1/Expression%20Analysis%20of%20Novel%20microRNAs%20in%20Rice.pdf
Sailaja, B and Mangrauthia, S K and Voleti, S R and Subrahmanyam, D and Brajendra, - and Ravindra Babu, V (2017) Expression Analysis of Novel microRNAs in Rice During High Temperature Stress. Bulletin of Environment, Pharmacology and Life Sciences, 6 (1). pp. 225-229. ISSN 2277-1808