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Molecular and phenotypic diversity among chickpea (Cicer arietinum) genotypes as a function of drought tolerance

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Relation http://oar.icrisat.org/10472/
http://dx.doi.org/10.1071/CP17284
10.1071/CP17284
 
Title Molecular and phenotypic diversity among chickpea (Cicer arietinum) genotypes as a function of drought tolerance
 
Creator Sachdeva, S
Bharadwaj, C
Sharma, V
Patil, B S
Soren, K R
Roorkiwal, M
Varshney, R K
Bhat, K V
 
Subject Abiotic Stress
Drought Tolerance
Molecular Biology
Chickpea
Genetics and Genomics
 
Description Diversity as a function of drought tolerance may be identified by morphological characters, and molecular tools used to find the most divergent genotypes for breeding programs for drought tolerance in future. The narrow genetic base of chickpea can be circumvented by using diverse lines in breeding programs. Forty chickpea genotypes were studied for their morphological and molecular diversity with an objective of identifying the most diverse drought-tolerant lines. In total, 90 alleles were detected with 3.6 alleles per locus. Polymorphism information content (PIC) values ranged from 0.155 to 0.782 with an average value of 0.4374 per locus. The size of amplified products ranged from 160 bp to 390 bp. Primer TA136 with eight alleles showed the highest PIC value of 0.7825, indicating its ability to differentiate the genotypes at molecular level. DARwin neighbour-joining tree analysis based on dissimilarity estimates was done for the molecular data and sequential agglomerative hierarchical non-overlapping (SAHN) grouping for the morphological data. It could clearly discriminate the tolerance and the sensitivity of genotypes. Two-dimensional principal coordinates analysis (PCoA) plot indicated good diversity for drought tolerance. The genetic similarity coefficients ranged from 0.115 (genotypes BGD72 to ICCV 5308) to 0.828 (genotypes ICCV 10316 to ICCV 92337).
 
Publisher CSIRO
 
Date 2018-02
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10472/1/Molecular%20and%20phenotypic%20diversity.pdf
Sachdeva, S and Bharadwaj, C and Sharma, V and Patil, B S and Soren, K R and Roorkiwal, M and Varshney, R K and Bhat, K V (2018) Molecular and phenotypic diversity among chickpea (Cicer arietinum) genotypes as a function of drought tolerance. Crop and Pasture Science, 69 (2). pp. 142-153. ISSN 1836-0947